先天性弓形虫病的诊断：在一项三方评估中，Platelia ELISA 法检测 IgA 抗体没有益处。
Diagnosis of Congenital Toxoplasmosis: No Benefit of IgA Antibody Detection by Platelia ELISA in a Tricentric Evaluation.
机构信息
Université de Montpellier, Centre National de la Recherche Scientifique, IRD, UMR MiVEGEC, Montpellier, France.
Département de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Montpellier, Montpellier, France.
出版信息
J Clin Microbiol. 2022 May 18;60(5):e0011622. doi: 10.1128/jcm.00116-22. Epub 2022 May 2.
The diagnostic accuracy of a commercial Toxoplasma gondii IgA antibody enzyme-linked immunosorbent assay (ELISA) was evaluated in the context of routine practice on 289 newborns with congenital toxoplasmosis (CT) and 220 healthy controls. The performance of this assay was compared to that of the current gold-standard test for anti- IgM detection, an immunosorbent agglutination assay (ISAGA). IgM and IgA sensitivity and specificity were assessed in cord and postnatal samples. The sensitivity of IgA detection by ELISA on all serum and peripheral blood samples was 60.56% and 56.52%, respectively, which is low compared with the sensitivity of IgM detection by ISAGA (73.26% on serum samples, 82.35% on peripheral blood). Adding the T. gondii IgA antibody ELISA to the diagnostic panel did not significantly increase the overall performance of the serological diagnosis based on IgM detection.
在对 289 例先天性弓形虫病（CT）新生儿和 220 例健康对照者进行常规检测的背景下，评估了一种商业化 Toxoplasma gondii IgA 抗体酶联免疫吸附试验（ELISA）的诊断准确性。该检测方法的性能与当前抗 IgM 检测的金标准检测方法——免疫吸附凝集试验（ISAGA）进行了比较。评估了 IgM 和 IgA 在脐带和产后样本中的敏感性和特异性。ELISA 检测所有血清和外周血样本的 IgA 敏感性分别为 60.56%和 56.52%，与 ISAGA 检测 IgM 的敏感性（血清样本 73.26%，外周血样本 82.35%）相比，敏感性较低。在 IgM 检测的基础上增加 T. gondii IgA 抗体 ELISA 检测并未显著提高血清学诊断的整体性能。
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