Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
RNA Biol. 2022;19(1):650-661. doi: 10.1080/15476286.2022.2067711. Epub 2021 Dec 31.
The nuclear pore complex (NPC) facilitates the trafficking of proteins and RNA between the nucleus and cytoplasm. The role of nucleoporins (Nups) in transport in the context of the NPC is well established, yet their function in tRNA export has not been fully explored. We selected several nucleoporins from different parts of the NPC to investigate their potential role in tRNA trafficking in . We show that while all of the nucleoporins studied are essential for cell viability, only TbNup62 and TbNup53a function in tRNA export. In contrast to homologs in yeast TbNup144 and TbNup158, which are part of the inner and outer ring of the NPC, have no role in nuclear tRNA trafficking. Instead, TbNup144 plays a critical role in nuclear division, highlighting the role of nucleoporins beyond nucleocytoplasmic transport. These results suggest that the location of nucleoporins within the NPC is crucial to maintaining various cellular processes.
核孔复合体（NPC）促进了细胞核和细胞质之间的蛋白质和 RNA 的运输。核孔蛋白（Nups）在 NPC 背景下的运输作用已经得到了很好的证实，但它们在 tRNA 输出中的功能尚未得到充分探索。我们选择了 NPC 不同部位的几种核孔蛋白，以研究它们在. 中的 tRNA 运输中的潜在作用。我们表明，虽然所有研究的核孔蛋白对于细胞活力都是必需的，但只有 TbNup62 和 TbNup53a 参与 tRNA 输出。与酵母中的同源物 TbNup144 和 TbNup158 不同，它们是 NPC 内外环的一部分，在核内 tRNA 运输中没有作用。相反，TbNup144 在核分裂中起着关键作用，突出了核孔蛋白在核质运输之外的作用。这些结果表明，NPC 内核孔蛋白的位置对于维持各种细胞过程至关重要。
