血浆中苯妥英的测定：使用新型放射免疫分析法、气相色谱法和酶免疫分析法的方法比较。

Determination of phenytoin in plasma: a comparison of procedures using a new radioimmunoassay, gas chromatography, and enzyme immunoassay.

作者信息

Stanley P E, Peikert M R

出版信息

Epilepsia. 1978 Jun;19(3):265-72. doi: 10.1111/j.1528-1157.1978.tb04489.x.

DOI:10.1111/j.1528-1157.1978.tb04489.x

Abstract

A new commercially available radioimmunoassay (RIA) procedure for the anticonvulsant drug phenytoin has been assessed and compared with a gas-liquid chromatographic (GLC) and an enzyme immunoassay (EMIT) procedure. One hundred and thirty-four plasma samples containing phenytoin were analyzed by the three methods and the results were found to be essentially independent of the procedure used and so would indicate similar clinical interpretation. The correlation coefficients were: RIA/GLC = 0.988; EMIT/RIA = 0.978; EMIT/GLC = 0.981. The cross reactivity of 33 drugs and related compounds was tested in the RIA and EMIT procedures and only a few very closely related substances showed significant cross reactivity. In addition, the three methods were compared with respect to the technical difficulties involved as well as time and cost for the assay of single and multiple samples.

摘要

一种新的可用于商业的抗惊厥药物苯妥英的放射免疫分析（RIA）方法已得到评估，并与气相色谱（GLC）法和酶免疫分析（EMIT）法进行了比较。采用这三种方法对134份含苯妥英的血浆样本进行了分析，结果发现基本上与所使用的方法无关，因此表明具有相似的临床解释。相关系数分别为：RIA/GLC = 0.988；EMIT/RIA = 0.978；EMIT/GLC = 0.981。在RIA和EMIT方法中测试了33种药物及相关化合物的交叉反应性，只有少数非常密切相关的物质显示出显著的交叉反应性。此外，还对这三种方法在技术难度、单样本和多样本检测的时间及成本方面进行了比较。