在自动动力学分析仪上评估用于测定五种主要抗癫痫药物的酶倍增免疫测定法。
Evaluation of EMIT methods for the determination of the five major antiepileptic drugs on an automated kinetic analyser.
作者信息
Malkus H, Dicesare J L, Meola J M, Pippenger C E, Ibanez J, Castro A
出版信息
Clin Biochem. 1978 Aug;11(4):139-42. doi: 10.1016/s0009-9120(78)90298-9.
We have evaluated the performance of enzyme-multipled immunoassay methods for the five major antiepileptic drugs on an automated system, the Perkin-Elmer Model KA-150 Kinetic Analyzer. The precision in the normal duplicate mode was found to be in the range of 6% to 10% for all five tests over a typical working day. All EMIT methods were compared to gas-liquid chromatographic procedures and, in addition, the phenytoin and phenobarbital assays were compared to a liquid-chromatographic method. The phenytoin assay was also compared to RIA and to a manual spectroscopic method. In general, most of the comparison studies resulted in acceptable correlation, although one gas chromatographic method did not correlate very well with the phenytoin and phenobarbital immunoassays.
我们在Perkin-Elmer KA-150型动力学自动分析仪上评估了酶放大免疫分析方法对五种主要抗癫痫药物的检测性能。在一个典型工作日内，所有五项检测在正常双份模式下的精密度范围为6%至10%。所有酶放大免疫分析方法都与气液色谱法进行了比较，此外，苯妥英和苯巴比妥检测还与液相色谱法进行了比较。苯妥英检测还与放射免疫分析法和手动光谱法进行了比较。总体而言，大多数比较研究得出了可接受的相关性，尽管有一种气相色谱法与苯妥英和苯巴比妥免疫分析的相关性不是很好。
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