Triastuti Asih, Pradana Dimas Adhi, Saputra Didit Erwin, Lianika Namira, Wicaksono Hilmy Ramadhan, Anisari Thessy Dewi, Widyarini Sitarina
Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, Indonesia.
Department of Pathology Faculty of Veterinary Medicine Universitas Gadjah Mada, Indonesia.
J Tradit Complement Med. 2021 Jul 28;12(3):219-224. doi: 10.1016/j.jtcme.2021.07.006. eCollection 2022 May.
has long been used for medical purposes in Indonesia. However, reports on the anti-arthritic activities of are limited.
The anti-arthritic properties of an n-hexane-insoluble fraction of dichloromethane extracts of (IPM) were evaluated using Complete Freund's Adjuvant (CFA)-induced arthritis induced in female Wistar rat by CFA. Diclofenac was used as a positive control. The volume of paw oedema, white blood cell count, lymphocytes, neutrophils, expression of TNF-α and Interleukin-6 and the histopathological features of the joint tissues were assessed to characterise IPM activity.
The IPM extract at doses of 280 and 420 mg/kg BW and diclofenac inhibited paw oedema by 15.70 %, 15.94 % and 19.71 % respectively. IPM also reduced the incidence of arthritis and arthritic index. Unlike untreated rats, animals treated with IPM showed a significant decrease in the number of neutrophils and decreased expression of TNF-α and Interleukin-6. Histopathological examination showed a reduction in the number of inflammatory cells and hyperplasia of the synovium after IPM treatment.
This study showed that displays anti-rheumatoid arthritis activity.
在印度尼西亚，[某种物质，原文未明确写出]长期以来一直用于医疗目的。然而，关于[该物质]抗关节炎活性的报道有限。
使用完全弗氏佐剂（CFA）诱导雌性Wistar大鼠患关节炎，以此评估[某种植物，原文未明确写出]二氯甲烷提取物的正己烷不溶部分（IPM）的抗关节炎特性。双氯芬酸用作阳性对照。评估爪部水肿体积、白细胞计数、淋巴细胞、中性粒细胞、肿瘤坏死因子-α（TNF-α）和白细胞介素-6的表达以及关节组织的组织病理学特征，以表征IPM的活性。
剂量为280和420毫克/千克体重的IPM提取物以及双氯芬酸分别抑制爪部水肿15.70%、15.94%和19.71%。IPM还降低了关节炎的发病率和关节炎指数。与未治疗的大鼠不同，用IPM治疗的动物中性粒细胞数量显著减少，TNF-α和白细胞介素-6的表达降低。组织病理学检查显示，IPM治疗后炎症细胞数量减少，滑膜增生减轻。
本研究表明，[某种物质，原文未明确写出]具有抗类风湿性关节炎活性。
