Zhao Jun, Liu Tao, Xu Fang, You Shuping, Xu Fang, Li Chenyang, Gu Zhengyi
Xinjiang Key Laboratory Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi, 830004, China.
Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi, 830011, China.
Pharmacogn Mag. 2016 Jul-Sep;12(47):178-83. doi: 10.4103/0973-1296.186346.
Twigs and leaves of Juniperus sabina L. have been traditionally used as the medicinal herb in China for the treatment of many ailments including rheumatoid arthritis (RA).
To confirm the therapeutic effect of total flavonoids from J. sabina (JSTF) on RA-induced by Complete Freund's Adjuvant (CFA) in rats.
Wistar rats (200 ± 20 g) were immunized by intradermal injection of 0.1 mL of CFA into the right hind metatarsal footpad. JSTF was administered orally at the dose of 125,250 and 500 mg/kg on 14 days after the induction of adjuvant arthritis. Tripterygium glycoside (20 mg/kg) was used as a positive control. Paw swelling, arthritic score, body weight loss, serum cytokines, inflammatory mediators, and histological change were measured.
We found that JSTF could ameliorate paw swelling of CFA rats, and significantly inhibit arthritic score (P < 0.05). The overproduction of tumor necrosis factor alpha and interleukin 1beta were remarkably suppressed in the serum of JSTF (125,500 mg/kg) treated rats (P < 0.05). Histopathological studies also showed a marked decrease of synovial inflammatory infiltration and synovial lining hyperplasia in the joints of JSTF-treated animals. Six flavonoids were isolated and from JSTF by various chromatographic methods and identified as follows: Catechin, quercitrin, isoquercitrin, isoscutellarein 7-O-β-D-xylopyranoside, isoscutellarein 7-O-β-D-xylopyranose-(1 → 3)-α-L-rhamnoside, and rutin.
These results suggest the potential therapeutically effect of JSTF as an anti-arthritis agent toward CFA-induced arthritis in rats, and verified therapeutic applications of J. sabina on RA in folk medicine.
Twigs and leaves of Juniperus sabina L. have been traditionally used as the medicinal herb in China for the treatment of rheumatoid arthritisJSTF could ameliorate paw swelling of CFA rats, and significantly inhibit arthritic scoreHistopathological studies showed a marked decrease of synovial inflammatory infiltration and synovial lining hyperplasia in the joints of JSTF-treated animalsSix flavonoids were isolated and from JSTF including: Catechin, quercitrin, isoquercitrin, isoscutellarein 7-O-β-D-xylopyranoside, isoscutellarein 7-O-β-D-xylopyranose-(1 → 3)-α-L- rhamnoside, and rutin. Abbreviations used: JSTF: Total flavonoids from Juniperus sabina; CFA: Complete Freund's Adjuvant; TG: Tripterygium glycoside; TNF-α: Tumor necrosis factor alpha; IL-1β: Interleukin 1beta; IL-6: Interleukin 6; H and E: Hematoxylin and eosin.
在中国，沙地柏的嫩枝和叶子传统上被用作草药，用于治疗包括类风湿性关节炎（RA）在内的多种疾病。
证实沙地柏总黄酮（JSTF）对弗氏完全佐剂（CFA）诱导的大鼠类风湿性关节炎的治疗效果。
将0.1 mL CFA皮内注射到Wistar大鼠（200±20 g）右后跖足垫进行免疫。在佐剂性关节炎诱导后14天，以125、250和500 mg/kg的剂量口服给予JSTF。雷公藤多苷（20 mg/kg）用作阳性对照。测量爪肿胀、关节炎评分、体重减轻、血清细胞因子、炎症介质和组织学变化。
我们发现JSTF可改善CFA大鼠的爪肿胀，并显著抑制关节炎评分（P<0.05）。在JSTF（125、500 mg/kg）治疗的大鼠血清中，肿瘤坏死因子α和白细胞介素1β的过量产生受到显著抑制（P<0.05）。组织病理学研究还显示，JSTF治疗动物关节中的滑膜炎症浸润和滑膜衬里增生明显减少。通过各种色谱方法从JSTF中分离出六种黄酮类化合物，鉴定如下：儿茶素、槲皮苷、异槲皮苷、异黄芩素7-O-β-D-吡喃木糖苷、异黄芩素7-O-β-D-吡喃木糖-（1→3）-α-L-鼠李糖苷和芦丁。
这些结果表明JSTF作为抗关节炎药物对CFA诱导的大鼠关节炎具有潜在的治疗作用，并验证了沙地柏在民间医学中对类风湿性关节炎的治疗应用。
在中国，沙地柏的嫩枝和叶子传统上被用作治疗类风湿性关节炎的草药。JSTF可改善CFA大鼠的爪肿胀，并显著抑制关节炎评分。组织病理学研究显示，JSTF治疗动物关节中的滑膜炎症浸润和滑膜衬里增生明显减少。从JSTF中分离出六种黄酮类化合物，包括：儿茶素、槲皮苷、异槲皮苷、异黄芩素7-O-β-D-吡喃木糖苷、异黄芩素7-O-β-D-吡喃木糖-（1→3）-α-L-鼠李糖苷和芦丁。使用的缩写：JSTF：沙地柏总黄酮；CFA：弗氏完全佐剂；TG：雷公藤多苷；TNF-α：肿瘤坏死因子α；IL-1β：白细胞介素1β；IL-6：白细胞介素6；H&E：苏木精和伊红。
