Fodor-Kardos Andrea, Kiss Ádám Ferenc, Monostory Katalin, Feczkó Tivadar
Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences Magyar Tudósok Körútja 2 H-1117 Budapest Hungary
Research Institute of Biomolecular and Chemical Engineering, University of Pannonia Egyetem u. 10 H-8200 Veszprém Hungary.
RSC Adv. 2020 Apr 22;10(27):15893-15900. doi: 10.1039/c9ra09928j. eCollection 2020 Apr 21.
Interferon-beta-1a (IFN-β-1a) can diminish the symptoms of relapsing-remitting multiple sclerosis. Herein, we prepared sustained drug delivery IFN-β-1a-loaded nanoparticles by a double emulsion solvent evaporation method. Bovine serum albumin (BSA) model drug was used to optimize the preparation of nanoparticles composed of four types of poly(lactic--glycolic acid) (PLGA) polymers and two pegylated PLGA (PEG-PLGA) polymers. optimization, selected PLGA and PEG-PLGA polymers were able to entrap IFN-β-1a with high encapsulation efficiency (>95%) and low size (145 nm and 163 nm, respectively). release kinetics of BSA and IFN-β showed similar tendency for PLGA and PEG-PLGA nanoparticles, respectively. Although the drug loaded nanoparticles did not show toxicity in hepatocyte cells, mild toxic effects such as pale kidney and pyelectasis were observed in the studies.
干扰素β-1a（IFN-β-1a）可减轻复发缓解型多发性硬化症的症状。在此，我们通过双乳液溶剂蒸发法制备了负载干扰素β-1a的持续药物递送纳米颗粒。使用牛血清白蛋白（BSA）模型药物优化了由四种聚乳酸-乙醇酸共聚物（PLGA）聚合物和两种聚乙二醇化PLGA（PEG-PLGA）聚合物组成的纳米颗粒的制备。经过优化，选定的PLGA和PEG-PLGA聚合物能够以高包封率（>95%）和小尺寸（分别为145纳米和163纳米）包载IFN-β-1a。BSA和IFN-β的释放动力学分别显示PLGA和PEG-PLGA纳米颗粒具有相似的趋势。尽管负载药物的纳米颗粒在肝细胞中未显示出毒性，但在研究中观察到了轻度毒性作用，如肾苍白和肾盂扩张。
