Pranitha P, Jhala M K, Bhanderi B B
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388 001 India.
Virusdisease. 2022 Mar;33(1):96-101. doi: 10.1007/s13337-022-00763-1. Epub 2022 Mar 25.
In present study, 50 nasal and conjunctival pooled swab samples from 48 dogs, one each from leopard and cat were collected, which were suspected for canine distemper from Anand and Ahmedabad districts during period of January-21 to June-21. Out of the 50 samples, 18% were positive for CD by Lateral flow assay (LFA). Using LFA, one each sample from leopard and cat samples were found negative for CD by LFA. Out of the total 50 (48 dogs, one each from leopard and cat) samples, 14 and 4% were positive by N-gene and H-gene based RT-PCR respectively. Comparative analysis of N and H genes, both the samples positive by H-gene based RT-PCR were also positive by N-gene based RT-PCR, which detected 5 more samples positive than H-gene based RT-PCR. Comparative analysis of N-gene based RT-PCR and LFA, relative sensitivity and specificity were 55.55% and 95.12% respectively. Comparative analysis of H-gene based RT-PCR and LFA relative sensitivity and specificity were 22.22 and 100% respectively.
在本研究中，于1月21日至6月21日期间，从阿南德和艾哈迈达巴德地区收集了48只狗的50份鼻拭子和结膜混合拭子样本，以及分别来自豹和猫的各一份样本，这些样本疑似患有犬瘟热。在这50份样本中，18%通过侧向流动分析法（LFA）检测为犬瘟热阳性。使用LFA检测，来自豹和猫的样本各有一份通过LFA检测为犬瘟热阴性。在总共50份（48只狗，豹和猫各一份）样本中，基于N基因和H基因的逆转录聚合酶链反应（RT-PCR）分别有14%和4%呈阳性。对N基因和H基因进行比较分析，基于H基因的RT-PCR检测呈阳性的两份样本，基于N基因的RT-PCR检测也呈阳性，且基于N基因的RT-PCR比基于H基因的RT-PCR多检测出5份阳性样本。对基于N基因的RT-PCR和LFA进行比较分析，相对灵敏度和特异性分别为55.55%和95.12%。对基于H基因的RT-PCR和LFA进行比较分析，相对灵敏度和特异性分别为22.22%和100%。
