Chen M, Xin T, Hou S, Lin W, Song W, Zhu H, Huang K, Jia H
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian, Beijing 100193, P.R. China.
College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang Street, Xuanwu, Nanjing 210095, Jiangsu Province, P.R. China.
Pol J Vet Sci. 2018 Sep;21(3):623-629. doi: 10.24425/124301.
Canine distemper virus (CDV) infects wild and domestic Canidae worldwide. The hemag- glutinin (H) gene has the highest genetic variation in the genome of this virus. Thus, the H gene is commonly used for lineage identification and genetic analyses. In order to study the genetic characteristics and pathogenicity of CDV strains prevalent in China, 132 samples were collected from domestic dogs with suspected CDV infection, 58 samples were confirmed to be positive, and the H gene was successfully amplified from 15 samples. The epidemic strain was identified as type Asia-1 and the novel mutations, A51T, V58I, R179K and D262N, were detected in this strain. Isolated strains, BJ16B53, BJ16B14, and BJ17B8, were used for an animal infection experiment in raccoon dogs. BJ16B53 and BJ16B14 were found to cause clinical symptoms, death, and exten- sive lesions in various organs. These results are expected to facilitate the development of effective strategies to monitor and control CDV infection in China.
犬瘟热病毒（CDV）感染全球范围内的野生和家养犬科动物。血凝素（H）基因在该病毒基因组中具有最高的遗传变异性。因此，H基因常用于谱系鉴定和遗传分析。为研究中国流行的CDV毒株的遗传特征和致病性，从疑似感染CDV的家犬中采集了132份样本，58份样本被确认为阳性，并且从15份样本中成功扩增出H基因。该流行毒株被鉴定为亚洲-1型，并且在该毒株中检测到新的突变A51T、V58I、R179K和D262N。分离出的毒株BJ16B53、BJ16B14和BJ17B8用于对貉进行动物感染实验。发现BJ16B53和BJ16B14会引发临床症状、导致死亡并在各个器官中造成广泛病变。这些结果有望促进制定有效的策略来监测和控制中国的CDV感染。
