Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.
Chemosphere. 2014 Jan;95:105-10. doi: 10.1016/j.chemosphere.2013.08.039. Epub 2013 Sep 10.
Bisphenol A (BPA) is a ubiquitous environmental contaminant in food products and aquatic ecosystems. Its endocrine and developmental toxicity presents a serious concern to human health and an effective high-throughput method for its detection is desirable. In this paper, water-soluble quantum dots (QDs) have been conjugated covalently with BPA antibodies and the conjugate has been utilized in a competitive fluorescence-linked immunoassay (FLISA). Cd-doped ZnO QDs were functionalized with poly(amidoamine) (PAMAM) dendrimers, as evidenced by ultraviolet absorption spectrum and fluorescence emission spectra analyses, and this led to their successful transfer into aqueous solution. Biological mass spectrometry demonstrated that the bisphenol A antibodies were successfully coupled to the water-soluble QDs, and the structures of these conjugates kept intact. The FLISA method allowed for BPA determination in a linear working range of 20.8-330.3 ng mL(-1) with the limit of detection (LOD) of 13.1 ng mL(-1). The recoveries of BPA from water samples were from 85.92% to 109.62%. In conclusion, a rapid and sensitive FLISA was developed by utilizing novel QD coupling method and validated for use in aqueous samples.
双酚 A (BPA) 是食品产品和水生生态系统中普遍存在的环境污染物。其内分泌和发育毒性对人类健康构成严重威胁，因此需要一种有效的高通量检测方法。本文将 BPA 抗体与水溶性量子点 (QDs) 通过共价键连接，并将该偶联物用于竞争性荧光酶联免疫吸附测定 (FLISA)。Cd 掺杂 ZnO QDs 通过聚酰胺-胺 (PAMAM) 树枝状大分子进行功能化，这可以通过紫外吸收光谱和荧光发射光谱分析得到证明，这导致它们成功地转移到水溶液中。生物质谱分析表明，双酚 A 抗体成功地偶联到水溶性 QDs 上，并且这些偶联物的结构保持完整。FLISA 方法允许在 20.8-330.3ng mL(-1) 的线性工作范围内测定 BPA，检测限 (LOD) 为 13.1ng mL(-1)。从水样中回收 BPA 的回收率为 85.92%至 109.62%。总之，利用新型 QD 偶联方法开发了一种快速灵敏的 FLISA，并验证了其在水样中的应用。
