Rathore Pankaj, Arora Indu, Rastogi Shweta, Akhtar Mohd, Singh Shruti, Samim Mohammed
Department of Chemistry, School of Chemical & Life Sciences, Jamia Hamdard New Delhi-62 India
Department of Biomedical Sciences, Shaheed Rajguru College, Delhi University New Delhi India.
RSC Adv. 2020 Jan 10;10(4):2241-2253. doi: 10.1039/c9ra08508d. eCollection 2020 Jan 8.
The effectiveness of curcumin in treating cerebral ischemia has been reported in recent studies. However, its mode of action is still not defined. The objective of the present study is to formulate collagen-curcumin nanocomposites which will work effectively against cerebral ischemia/reperfusion injury. Ischemic injury is followed by inflammatory damage and oxidative stress, which together contribute a lot in the pathogenesis of cerebral ischemia and may be considered a good target for treatment. The present study focused on examining the effectiveness of collagen-curcumin nanocomposites stabilized by increasing the degree of crosslinking in reducing oxidative stress associated with brain injury resulting from cerebral ischemia. The collagen nanoparticles were prepared by conjugating collagen on the surface of Tween©80 micelles, and further stabilizing them using crosslinkers. The effectiveness of the prepared nanocomposite was validated by performing infarct analysis followed by biochemical, behavioral, histopathological and immunohistochemical studies. The outcomes of this study are promising for the use of collagen-curcumin nanocomposites in showing neuroprotective potential in treating ischemic injury.
近期研究报道了姜黄素治疗脑缺血的有效性。然而，其作用模式仍未明确。本研究的目的是制备胶原蛋白-姜黄素纳米复合材料，该材料将有效对抗脑缺血/再灌注损伤。缺血性损伤之后会出现炎症损伤和氧化应激，它们在脑缺血的发病机制中起很大作用，可被视为一个良好的治疗靶点。本研究着重考察通过增加交联度来稳定的胶原蛋白-姜黄素纳米复合材料在减轻与脑缺血所致脑损伤相关的氧化应激方面的有效性。通过将胶原蛋白缀合在吐温®80胶束表面制备胶原蛋白纳米颗粒，并使用交联剂进一步使其稳定。通过进行梗死分析以及随后的生化、行为、组织病理学和免疫组织化学研究，验证了所制备纳米复合材料的有效性。本研究结果对于胶原蛋白-姜黄素纳米复合材料在治疗缺血性损伤中展现神经保护潜力的应用而言很有前景。
