Le Thi H, Phan An H T, Le Khoa C M, Phan Thy D U, Nguyen Khoi T
School of Biotechnology, International University, Vietnam National University Ho Chi Minh City Vietnam +84(8)3724 4271 +84(8)3724 4270.
School of Chemical Engineering, The University of Queensland Brisbane QLD 4072 Australia
RSC Adv. 2021 Oct 25;11(55):34440-34448. doi: 10.1039/d1ra04983f.
The application of ultrafine bubbles as drug carriers in drug delivery is still in its developmental stage; it is important to obtain a thorough understanding of the factors affecting the formation and stability of drug carrier matrices. In this study, the polyethylene glycol (PEG)-conjugated human serum albumin (HSA)-based ultrafine bubble simulating the physiological electrolyte concentration in human blood (154 mM) for quercetin delivery was investigated. Optical absorbance measurements, surface tension measurements, and fluorescence laser imaging were also employed to assess the plausibility of polymer-conjugated albumin-stabilized ultrafine bubbles in drug loading and drug release. The incorporation of PEG/HSA into the system illustrated a significant enhancement in the matrix's stability as confirmed by surface tension measurements and drug-loading efficiency achieving approximately 90%. In addition, drug release was performed by the application of high-frequency ultrasound, indicating more than 40% of the loaded quercetin was astonishingly liberated within 5 minutes of exposure.
将超细气泡作为药物载体应用于药物递送仍处于发展阶段；全面了解影响药物载体基质形成和稳定性的因素非常重要。在本研究中，研究了基于聚乙二醇（PEG）共轭人血清白蛋白（HSA）的超细气泡，其模拟人体血液中的生理电解质浓度（154 mM）用于槲皮素递送。还采用了光吸收测量、表面张力测量和荧光激光成像来评估聚合物共轭白蛋白稳定的超细气泡在药物负载和药物释放方面的合理性。通过表面张力测量和达到约90%的载药效率证实，将PEG/HSA加入系统可显著提高基质的稳定性。此外，通过应用高频超声进行药物释放，结果表明在暴露5分钟内，超过40%的负载槲皮素惊人地释放出来。
