Tomita T, Watanabe I, Rengachary S S
Hum Pathol. 1987 Apr;18(4):367-74. doi: 10.1016/s0046-8177(87)80167-3.
Growth hormone (GH)- and prolactin (Prl)-producing pituitary adenomas were studied with immunoelectron microscopy using protein A-gold complex, and were compared with the normal pituitary gland. GH-producing cells were readily identifiable by numerous, uniformly dense, round secretory granules in both adenomas and normal pituitary gland. In contrast, Prl secretory granules in normal pituitary gland were much larger in size than the scarce, smaller, secretory granules of Prl-producing adenomas. Thus immunoelectron microscopic identification of Prl is more valuable for prolactinoma. With more specific antigens available as tumor markers, immunoelectron microscopy appears to be a powerful tool for tumor diagnosis.
利用蛋白A-金复合物免疫电子显微镜技术对生长激素（GH）分泌型和催乳素（Prl）分泌型垂体腺瘤进行了研究，并与正常垂体进行了比较。在腺瘤和正常垂体中，通过大量均匀致密的圆形分泌颗粒很容易识别出分泌GH的细胞。相比之下，正常垂体中的Prl分泌颗粒比分泌Prl的腺瘤中稀少、较小的分泌颗粒要大得多。因此，免疫电子显微镜对Prl的鉴定对催乳素瘤更有价值。随着有更多特异性抗原可作为肿瘤标志物，免疫电子显微镜似乎是肿瘤诊断的有力工具。
