Elsayis Asmaa, Hassan Sahar W M, Ghanem Khaled M, Khairy Heba
National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Front Microbiol. 2022 Apr 13;13:871394. doi: 10.3389/fmicb.2022.871394. eCollection 2022.
The marine ecosystem is a complex niche with unique environmental circumstances. Microbial communities from the sea are one of the main origins of compounds with tremendous capabilities. Marine yeasts have the ability to produce secondary metabolites that are architecturally distinct from those found in terrestrial species. Melanin pigment synthesized by marine halotolerant black yeast AS1 isolated from Mediterranean salt lakes in Alexandria, Egypt was found to exert a radical scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) with an IC of 61.38 μg/ml. Furthermore, it showed no cytotoxicity toward human skin fibroblast cell line (HSF) with an IC value above 0.1 mg/ml. The antimicrobial capability of the pigment was revealed against the tested number of bacterial and fungal strains with the highest inhibition zone of 25 mm against sp. and a growth inhibition percentage up to 63.6% against . From an environmental impact point of view, the pigment disclosed a heavy metal removal efficiency of 85.7, 84.8, and 81.5% for Pb, Cd, and Ni, respectively, at 100 mg/L metal concentration. The previously mentioned results suggested melanin from AS1 as a promising biocompatible candidate in various medical, cosmetics, pharmaceutical, and environmental applications.
海洋生态系统是一个具有独特环境条件的复杂生态位。海洋微生物群落是具有巨大功能的化合物的主要来源之一。海洋酵母能够产生结构上与陆地物种不同的次生代谢产物。从埃及亚历山大港地中海盐湖分离出的耐盐黑色海洋酵母AS1合成的黑色素对2,2-二苯基-1-苦基肼（DPPH）具有自由基清除作用，其半数抑制浓度（IC）为61.38μg/ml。此外，它对人皮肤成纤维细胞系（HSF）没有细胞毒性，半数抑制浓度值高于0.1mg/ml。该色素对测试的多种细菌和真菌菌株具有抗菌能力，对某菌株的最大抑菌圈为25mm，对另一菌株的生长抑制率高达63.6%。从环境影响的角度来看，在金属浓度为100mg/L时，该色素对铅、镉和镍的重金属去除效率分别为85.7%、84.8%和81.5%。上述结果表明，来自AS1的黑色素在各种医学、化妆品、制药和环境应用中是一种有前景的生物相容性候选物。
