Li Mengting, Wang Zhenyu, Zhou Miao, Zhang Chong, Zhi Kaiqi, Liu Shuli, Sun Xiujuan, Wang Zhi, Liu Jinle, Liu Dong
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
School of Life Sciences, Zhengzhou University, Zhengzhou, China.
Front Microbiol. 2022 Apr 12;13:855059. doi: 10.3389/fmicb.2022.855059. eCollection 2022.
Increasing demand for recombinant proteins necessitates efficient protein production processes. In this study, a continuous process for human epidermal growth factor (hEGF) secretion by was developed by taking advantage of biofilm formation. Genes , , and that have proved to facilitate biofilm formation and some genes , , , and potentially involved in biofilm formation were examined for their effects on hEGF secretion as well as biofilm formation. Finally, biofilm-based fermentation processes were established, which demonstrated the feasibility of continuous production of hEGF with improved efficiency. The best result was obtained from -disruption that showed a 28% increase in hEGF secretion over the BL21(DE3) wild strain, from 24 to 32 mg/L. Overexpression of also showed great potential in continuous immobilized fermentation. Overall, the biofilm engineering here represents an effective strategy to improve hEGF production and can be adapted to produce more recombinant proteins in future.
对重组蛋白的需求不断增加，这就需要高效的蛋白质生产工艺。在本研究中，利用生物膜形成开发了一种人表皮生长因子（hEGF）连续分泌的工艺。研究了已被证明有助于生物膜形成的基因、和以及一些可能参与生物膜形成的基因、、和对hEGF分泌以及生物膜形成的影响。最后，建立了基于生物膜的发酵工艺，证明了连续高效生产hEGF的可行性。破坏获得了最佳结果，hEGF分泌量比BL21(DE3)野生菌株增加了28%，从24毫克/升增加到32毫克/升。的过表达在连续固定化发酵中也显示出巨大潜力。总体而言，这里的生物膜工程是提高hEGF产量的有效策略，未来可用于生产更多重组蛋白。
