Zhao Cheng, Kao Xiaoming, Wang Zhiwei, Liu Qinjie, Wu Jie, Hu Qiongyuan, Wu Xiuwen, Ren Jianan
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University Nanjing 210002 China
Lab for Trauma and Surgical Infection, Jinling Hospital Nanjing China.
RSC Adv. 2020 Mar 3;10(15):9126-9132. doi: 10.1039/c9ra08721d. eCollection 2020 Feb 27.
Biomimetic enzyme barrier (BEB) encapsulated microcapsules with alginate shells were fabricated with a microfluidic electrospray approach for preventing intestine-derived LPS induced diseases. As the alginate shells could protect the contents in gastric juice and release them in the intestine, the inner BEB could form a consecutive immune barrier on the surface of the intestine during the release. Through combining BEB with alkaline phosphatase, the immune barrier could degrade and prevent the permeation of lipopolysaccharide, which enhanced the intestinal barrier function. Thus, the BEB microcapsules were imparted with outstanding ability in preventing intestine-derived LPS induced diseases. Based on an study, we demonstrated that this BEB microcapsule could effectively protect organ function, restore intestinal barrier integrity, prevent the permeation of LPS and alleviate inflammation. Therefore, the generated microcapsules have potential for clinical applications.
采用微流控电喷雾法制备了具有藻酸盐外壳的仿生酶屏障（BEB）包封微胶囊，用于预防肠道源性脂多糖诱导的疾病。由于藻酸盐外壳可以保护内容物不被胃液破坏，并在肠道中释放，内部的BEB在释放过程中可以在肠道表面形成连续的免疫屏障。通过将BEB与碱性磷酸酶结合，免疫屏障可以降解并阻止脂多糖的渗透，从而增强肠道屏障功能。因此，BEB微胶囊在预防肠道源性脂多糖诱导的疾病方面具有出色的能力。基于一项研究，我们证明这种BEB微胶囊可以有效保护器官功能，恢复肠道屏障完整性，阻止脂多糖的渗透并减轻炎症。因此，所制备的微胶囊具有临床应用潜力。
