日粮绿原酸对脂多糖诱导免疫应激肉鸡肠道屏障功能和炎症反应的影响。
Effects of dietary chlorogenic acid on intestinal barrier function and the inflammatory response in broilers during lipopolysaccharide-induced immune stress.
机构信息
Department of Animal Physiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China; Innovative Research Team of Livestock Intelligent Breeding and Equipment, Longmen Laboratory, Luoyang 471023, China.
Department of Animal Physiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China; Henan International Joint Laboratory of Animal Welfare and Health Breeding, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
出版信息
Poult Sci. 2023 May;102(5):102623. doi: 10.1016/j.psj.2023.102623. Epub 2023 Mar 4.
Immune stress exerts detrimental effects on growth performance and intestinal barrier function during intensive animal production with ensuing serious economic consequences. Chlorogenic acid (CGA) is used widely as a feed additive to improve the growth performance and intestinal health of poultry. However, the effects of dietary CGA supplementation on amelioration of the intestinal barrier impairment caused by immune stress in broilers are unknown. This study investigated the effects of CGA on growth performance, intestinal barrier function, and the inflammatory response in lipopolysaccharide (LPS) mediated immune-stressed broilers. Three hundred and twelve 1-day-old male Arbor Acres broilers were divided randomly into 4 groups with 6 replicates of thirteen broilers. The treatments included: i) saline group: broilers injected with saline and fed with basal diet; ii) LPS group: broilers injected with LPS and fed with basal diet; iii) CGA group: broilers injected with saline and feed supplemented with CGA; and iv) LPS+CGA group: broilers injected with LPS and feed supplemented with CGA. Animals in the LPS and LPS+CGA groups were injected intraperitoneally with an LPS solution prepared with saline from 14 d of age for 7 consecutive days, whereas broilers in the other groups were injected only with saline. LPS induced a decrease in feed intake of broilers during the stress period, but CGA effectively alleviated this decrease. Moreover, CGA inhibited the reduction of villus height and improved the ratio of villus height to crypt depth in the duodenum of broilers 24 and 72 h after LPS injection. In addition, dietary CGA supplementation significantly restored the expression of cation-selective and channel-forming Claudin2 protein 2 h after LPS injection in the ileum. LPS enhanced the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the small intestine, but this enhancement was blocked by CGA supplementation. The expression of interleukin-10 (IL-10) increased with LPS injection and CGA promoted the production of IL-10. CGA addition downregulated the expression of intestinal interleukin-6 (IL-6) of broilers under normal rearing conditions. However, CGA supplementation upregulated the expression of IL-6 of broilers 72 h after LPS injection. The data demonstrate that dietary supplementation with CGA alleviates intestinal barrier damage and intestinal inflammation induced by LPS injection during immune stress thereby improving growth performance of broilers.
免疫应激在集约化动物生产中对生长性能和肠道屏障功能产生不利影响，从而造成严重的经济后果。绿原酸（CGA）被广泛用作饲料添加剂，以改善家禽的生长性能和肠道健康。然而，日粮 CGA 补充对缓解脂多糖（LPS）介导的免疫应激肉鸡肠道屏障损伤的影响尚不清楚。本研究探讨了 CGA 对 LPS 介导的免疫应激肉鸡生长性能、肠道屏障功能和炎症反应的影响。将 312 只 1 日龄雄性爱宝肉鸡随机分为 4 组，每组 13 只肉鸡，每组 6 个重复。处理包括：i）盐水组：注射生理盐水并饲喂基础日粮的肉鸡；ii）LPS 组：注射 LPS 并饲喂基础日粮的肉鸡；iii）CGA 组：注射生理盐水并饲喂添加 CGA 的日粮的肉鸡；iv）LPS+CGA 组：注射 LPS 并饲喂添加 CGA 的日粮的肉鸡。LPS 和 LPS+CGA 组的动物从 14 日龄开始连续 7 天每天腹腔内注射用生理盐水配制的 LPS 溶液，而其他组的动物仅注射生理盐水。LPS 应激期间导致肉鸡采食量下降，但 CGA 有效缓解了这种下降。此外，CGA 抑制了 LPS 注射后 24 和 72 小时肉鸡十二指肠绒毛高度的降低，并改善了绒毛高度与隐窝深度的比值。此外，日粮 CGA 补充显著恢复了 LPS 注射后 2 小时空肠中阳离子选择性和通道形成 Claudin2 蛋白的表达。LPS 增强了小肠中肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的表达，但 CGA 补充抑制了这种增强。IL-10 的表达随着 LPS 注射而增加，CGA 促进了 IL-10 的产生。CGA 补充在正常饲养条件下下调了肉鸡肠道白细胞介素-6（IL-6）的表达，但 LPS 注射后 72 小时 CGA 补充上调了肉鸡 IL-6 的表达。数据表明，日粮 CGA 补充缓解了免疫应激期间 LPS 注射引起的肠道屏障损伤和肠道炎症，从而改善了肉鸡的生长性能。
Zotero 插件