诱导型 piggyBac 转座子系统在人多能干细胞中高效基因过表达的方案。
Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells.
机构信息
Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325015, China.
出版信息
STAR Protoc. 2022 Apr 13;3(2):101296. doi: 10.1016/j.xpro.2022.101296. eCollection 2022 Jun 17.
In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of and/or in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs. For complete details on the use and execution of this protocol, please refer to Quan et al. (2021).
在人类多能干细胞（hPSCs）中，传统的基因过表达方法效率低，且通常很繁琐。在这里，我们提供了一种相对简单的方法，使用 Dox 诱导型 PiggyBac 转座子系统进行基因过表达。我们以 H1 胚胎干细胞（H1 ESCs）中过表达 和/或 为例，详细介绍了该方法的步骤。我们的方案可适用于各种 hPSCs 中的任何目的基因。如需了解本方案的详细使用和执行方法，请参考 Quan 等人（2021）。
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