Department of Biosciences and Nutrition, Karolinska Institutet, NEO, 14183 Huddinge, Sweden.
Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland.
STAR Protoc. 2022 Apr 19;3(2):101338. doi: 10.1016/j.xpro.2022.101338. eCollection 2022 Jun 17.
Chromosome conformation capture combined with high-throughput sequencing (4C-seq) is a powerful tool to map genomic DNA regions that communicate with a specific locus of interest such as functional single-nucleotide polymorphism (SNPs)-containing regions. This protocol describes detailed steps to perform 4C-seq in mouse macrophage RAW264.7 cells, starting from the primer design based on cistrome and epigenome data, sample processing, and to the bioinformatics analysis. For complete details on the use and execution of this protocol, please refer to Huang et al. (2021).
染色体构象捕获结合高通量测序（4C-seq）是一种强大的工具，可用于绘制与特定感兴趣基因座（如含有功能单核苷酸多态性（SNP）的区域）相互作用的基因组 DNA 区域图谱。本方案描述了在小鼠巨噬细胞 RAW264.7 细胞中进行 4C-seq 的详细步骤，从基于顺式作用元件和表观基因组数据的引物设计、样品处理到生物信息学分析。有关本方案使用和执行的完整详细信息，请参阅 Huang 等人（2021 年）。
