McCord Rachel Patton, Kaplan Noam, Giorgetti Luca
Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, USA.
Department of Physiology, Biophysics and Systems Biology, Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel.
Mol Cell. 2020 Feb 20;77(4):688-708. doi: 10.1016/j.molcel.2019.12.021. Epub 2020 Jan 27.
Rapidly developing technologies have recently fueled an exciting era of discovery in the field of chromosome structure and nuclear organization. In addition to chromosome conformation capture (3C) methods, new alternative techniques have emerged to study genome architecture and biological processes in the nucleus, often in single or living cells. This sets an unprecedented stage for exploring the mechanisms that link chromosome structure and biological function. Here we review popular as well as emerging approaches to study chromosome organization, focusing on the contribution of complementary methodologies to our understanding of structures revealed by 3C methods and their biological implications, and discuss the next technical and conceptual frontiers.
快速发展的技术最近推动了染色体结构和核组织领域令人兴奋的发现时代。除了染色体构象捕获（3C）方法外，新的替代技术也已出现，用于研究细胞核中的基因组结构和生物学过程，这些研究通常在单个或活细胞中进行。这为探索连接染色体结构和生物学功能的机制搭建了一个前所未有的平台。在这里，我们综述了研究染色体组织的常用方法以及新兴方法，重点关注互补方法对我们理解3C方法揭示的结构及其生物学意义的贡献，并讨论下一个技术和概念前沿。
