根癌土壤杆菌质粒pTiC58的virE基因座的特征分析
Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58.
作者信息
Hirooka T, Rogowsky P M, Kado C I
出版信息
J Bacteriol. 1987 Apr;169(4):1529-36. doi: 10.1128/jb.169.4.1529-1536.1987.
Abstract
The virE locus that is responsible for the efficiency of infection by Agrobacterium tumefaciens (T. Hirooka and C. Kado, J. Bacteriol. 168:237-243, 1986) is located next to the right boundary of the virulence (Vir) region of the nopaline plasmid pTiC58. This locus is very similar to the virE locus of octopine type Ti plasmids on the basis of nucleotide and amino acid sequence comparisons as well as genetic complementation analyses. The nucleotide sequence of virE revealed three open reading frames, arranged as an operon, with a potential coding capacity for proteins of 9, 7.1, and 63.5 kilodaltons. The promoter region of virE was analyzed by using gene fusions to promoterless cat and lux genes. Two different promoters were detected, one which operates in A. tumefaciens and one which operates in Escherichia coli. virE is transcribed from left to right toward the T region. In A. tumefaciens, the expression of virE was induced by acetosyringone and required the presence of pTiC58.
摘要
负责根癌土壤杆菌感染效率的virE基因座（T. Hirooka和C. Kado，《细菌学杂志》168:237 - 243，1986年）位于胭脂碱型质粒pTiC58的毒力（Vir）区域右边界附近。基于核苷酸和氨基酸序列比较以及遗传互补分析，该基因座与章鱼碱型Ti质粒的virE基因座非常相似。virE的核苷酸序列揭示了三个开放阅读框，它们作为一个操纵子排列，具有编码9、7.1和63.5千道尔顿蛋白质的潜在能力。通过使用与无启动子的cat和lux基因的基因融合来分析virE的启动子区域。检测到两个不同的启动子，一个在根癌土壤杆菌中起作用，另一个在大肠杆菌中起作用。virE从左向右朝着T区域转录。在根癌土壤杆菌中，virE的表达由乙酰丁香酮诱导，并且需要pTiC58的存在。
相似文献
1
Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58.根癌土壤杆菌质粒pTiC58的virE基因座的特征分析
J Bacteriol. 1987 Apr;169(4):1529-36. doi: 10.1128/jb.169.4.1529-1536.1987.
2
Location of the right boundary of the virulence region on Agrobacterium tumefaciens plasmid pTiC58 and a host-specifying gene next to the boundary.根癌土壤杆菌质粒pTiC58上毒性区域右边界的位置以及边界旁的一个宿主特异性基因。
J Bacteriol. 1986 Oct;168(1):237-43. doi: 10.1128/jb.168.1.237-243.1986.
3
Characterization of the virE operon of the Agrobacterium Ti plasmid pTiA6.根癌土壤杆菌Ti质粒pTiA6的virE操纵子的特性分析
Nucleic Acids Res. 1987 Jan 26;15(2):825-37. doi: 10.1093/nar/15.2.825.
4
Genetic analysis of the virE operon of the Agrobacterium Ti plasmid pTiA6.根癌土壤杆菌Ti质粒pTiA6的virE操纵子的遗传分析
J Bacteriol. 1988 Apr;170(4):1430-7. doi: 10.1128/jb.170.4.1430-1437.1988.
5
Dual control of Agrobacterium tumefaciens Ti plasmid virulence genes.根癌土壤杆菌Ti质粒毒性基因的双重控制
J Bacteriol. 1987 Nov;169(11):5113-8. doi: 10.1128/jb.169.11.5113-5118.1987.
6
The right end of the vir region of an octopine-type Ti plasmid contains four new members of the vir regulon that are not essential for pathogenesis.章鱼碱型Ti质粒vir区域的右端包含四个对致病作用并非必需的vir调节子新成员。
J Bacteriol. 2000 Mar;182(6):1774-8. doi: 10.1128/JB.182.6.1774-1778.2000.
7
Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58.根癌土壤杆菌质粒pTiC58的vir基因调控
J Bacteriol. 1987 Nov;169(11):5101-12. doi: 10.1128/jb.169.11.5101-5112.1987.
8
Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG, and vir box from the same Ti plasmid.根癌农杆菌中高效的vir基因诱导需要来自同一Ti质粒的virA、virG和vir盒。
J Bacteriol. 2001 Jul;183(13):4079-89. doi: 10.1128/JB.183.13.4079-4089.2001.
9
Characteristics of the nopaline catabolic plasmid in Agrobacterium strains K84 and K1026 used for biological control of crown gall disease.用于防治根癌病的土壤杆菌菌株K84和K1026中胭脂碱分解代谢质粒的特性
Plasmid. 1990 Mar;23(2):126-37. doi: 10.1016/0147-619x(90)90031-7.
10
A comparison of virulence determinants in an octopine Ti plasmid, a nopaline Ti plasmid, and an Ri plasmid by complementation analysis of Agrobacterium tumefaciens mutants.通过根癌土壤杆菌突变体的互补分析比较章鱼碱型Ti质粒、胭脂碱型Ti质粒和Ri质粒中的毒力决定因素。
Plasmid. 1984 May;11(3):195-205. doi: 10.1016/0147-619x(84)90026-x.
引用本文的文献
1
Enhanced stable expression of aVibrio luciferase under the control of the Ω-translational enhancer in transgenic plants.在转基因植物中，Ω 翻译增强子控制下的 Vibrio 荧光素酶的增强稳定表达。
World J Microbiol Biotechnol. 1992 Nov;8(6):638-44. doi: 10.1007/BF01238805.
2
Ubiquitination during plant immune signaling.植物免疫信号传导过程中的泛素化作用
Plant Physiol. 2012 Sep;160(1):15-27. doi: 10.1104/pp.112.199281. Epub 2012 Jun 11.
3
A plethora of virulence strategies hidden behind nuclear targeting of microbial effectors.大量隐藏在微生物效应物核靶向背后的毒力策略。
Front Plant Sci. 2011 Dec 27;2:104. doi: 10.3389/fpls.2011.00104. eCollection 2011.
4
Interactions of bacterial proteins with host eukaryotic ubiquitin pathways.细菌蛋白与宿主真核泛素途径的相互作用。
Front Microbiol. 2011 Jul 4;2:143. doi: 10.3389/fmicb.2011.00143. eCollection 2011.
5
Exploitation of Host Polyubiquitination Machinery through Molecular Mimicry by Eukaryotic-Like Bacterial F-Box Effectors.真核样细菌F-Box效应蛋白通过分子模拟对宿主多聚泛素化机制的利用
Front Microbiol. 2010 Nov 11;1:122. doi: 10.3389/fmicb.2010.00122. eCollection 2010.
6
VIP1: linking Agrobacterium-mediated transformation to plant immunity?VIP1：将农杆菌介导的转化与植物免疫联系起来？
Plant Cell Rep. 2010 Aug;29(8):805-12. doi: 10.1007/s00299-010-0870-4. Epub 2010 May 15.
7
Agrobacterium induces expression of a host F-box protein required for tumorigenicity.农杆菌诱导宿主 F-box 蛋白表达，该蛋白是肿瘤发生所必需的。
Cell Host Microbe. 2010 Mar 18;7(3):197-209. doi: 10.1016/j.chom.2010.02.009.
8
Resistance to crown gall disease in transgenic grapevine rootstocks containing truncated virE2 of Agrobacterium.含截短的农杆菌 virE2 的转基因葡萄砧木对冠瘿病的抗性。
Transgenic Res. 2010 Dec;19(6):949-58. doi: 10.1007/s11248-010-9373-x. Epub 2010 Feb 25.
9
Evidence for stable transformation of wheat by floral dip in Agrobacterium tumefaciens.通过农杆菌介导的花浸染法实现小麦稳定转化的证据。
Plant Cell Rep. 2009 Jun;28(6):903-13. doi: 10.1007/s00299-009-0696-0. Epub 2009 Mar 24.
10
Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis nuclear protein VIP1.通过过量表达拟南芥核蛋白VIP1提高植物对农杆菌感染的敏感性。
Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10435-40. doi: 10.1073/pnas.162304099. Epub 2002 Jul 17.
本文引用的文献
1
A plant cell factor induces Agrobacterium tumefaciens vir gene expression.一种植物细胞因子诱导根癌农杆菌 vir 基因表达。
Proc Natl Acad Sci U S A. 1986 Jan;83(2):379-83. doi: 10.1073/pnas.83.2.379.
2
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.用于革兰氏阴性菌的广宿主范围DNA克隆系统：苜蓿根瘤菌基因文库的构建
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51. doi: 10.1073/pnas.77.12.7347.
3
Rapid procedure for detection and isolation of large and small plasmids.用于检测和分离大小质粒的快速方法。
J Bacteriol. 1981 Mar;145(3):1365-73. doi: 10.1128/jb.145.3.1365-1373.1981.
4
Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens.吲哚乙酸互补作用及其与根癌土壤杆菌Ti质粒上宿主范围决定基因的关系。
Mol Gen Genet. 1982;188(3):425-32. doi: 10.1007/BF00330044.
5
trans-Acting virulence functions of the octopine Ti plasmid from Agrobacterium tumefaciens.根癌土壤杆菌章鱼碱型Ti质粒的反式作用毒力功能
J Bacteriol. 1984 May;158(2):754-6. doi: 10.1128/jb.158.2.754-756.1984.
6
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
7
Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region.根癌土壤杆菌Ti质粒毒性区域突变体的遗传互补
Mol Gen Genet. 1984;193(1):1-7. doi: 10.1007/BF00327406.
8
Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.氯霉素抗性基因盒的构建与特性分析：染色体外元件转录图谱绘制的新方法。
Gene. 1982 Dec;20(2):305-16. doi: 10.1016/0378-1119(82)90048-8.
9
Units of genetic expression in the virulence region of a plant tumor-inducing plasmid of Agrobacterium tumefaciens.根癌土壤杆菌致瘤质粒毒性区域中的基因表达单位。
Mol Gen Genet. 1982;188(3):418-24. doi: 10.1007/BF00330043.
10
T-DNA of the Agrobacterium Ti and Ri plasmids.根癌农杆菌Ti质粒和发根农杆菌Ri质粒的T-DNA
Annu Rev Genet. 1982;16:357-84. doi: 10.1146/annurev.ge.16.120182.002041.
Zotero 插件