Wu Kuo-Hui, Huang Wen-Chien, Chang Shu-Chen, Shyu Rong-Hwa
Department of Chemical and Materials Engineering, Chung Cheng Institute of Technology, National Defense University Taoyuan 33551 Taiwan
Applied Zoology Division, Taiwan Agricultural Research Institute Taichung 41362 Taiwan.
RSC Adv. 2022 Apr 29;12(21):13035-13044. doi: 10.1039/d2ra01654k. eCollection 2022 Apr 28.
A colloidal silver nanoparticle (AgNP)-based lateral flow immunoassay (LFIA) was evaluated in terms of the rapid detection of profenofos (PEO) pesticide residue in vegetables. Colloidal AgNPs, of a diameter of approximately 20 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order to improve their stability and dispersion. An anti-profenofos polyclonal antibody (pAb) was successfully immobilized on the surface of the AgNPs by ionic interaction and characterized using UV-vis, SEM, TEM, FTIR and XPS analyses. Surface modification of Ag-pAb conjugates of varying pH, pAb content and cross-reactivity was employed to design and prepare labels for use in an LFIA to examine whether these factors affect the performance of the assay. The visible detection limit and optical detection limit of the PEO test strip were 0.20 and 0.01 ppm, respectively, in PEO standard solution. This assay showed no cross-reaction with omethoate, methamidophos or pyraclofos. Finally, the PEO test strip was effectively applied for the detection of PEO in liquid vegetables A and B, with optical detection limits of 0.09 and 0.075 ppm, respectively.
评估了一种基于胶体银纳米颗粒（AgNP）的侧向流动免疫分析（LFIA）用于快速检测蔬菜中丙溴磷（PEO）农药残留的能力。直径约为20 nm的胶体AgNP用柠檬酸三钠脱水物（TSC）进行表面改性，以提高其稳定性和分散性。通过离子相互作用将抗丙溴磷多克隆抗体（pAb）成功固定在AgNP表面，并使用紫外可见光谱、扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱和X射线光电子能谱分析对其进行表征。采用不同pH值、pAb含量和交叉反应性的Ag-pAb缀合物表面改性来设计和制备用于LFIA的标记物，以研究这些因素是否会影响分析性能。在PEO标准溶液中，PEO试纸条的可见检测限和光学检测限分别为0.20 ppm和0.01 ppm。该分析方法与氧乐果、甲胺磷或吡唑磷没有交叉反应。最后，PEO试纸条有效地应用于检测液体蔬菜A和B中的PEO，光学检测限分别为0.09 ppm和0.075 ppm。
