Huang Wen-Chien, Wu Kuo-Hui, Hung Hao-Cheng, Wang Je-Chuang, Chang Shu-Chen
J Nanosci Nanotechnol. 2018 Oct 1;18(10):7190-7196. doi: 10.1166/jnn.2018.16020.
Magnetite nanoparticles (MNPs) Fe3O4 and r-Fe2O3 were surface-modified with poly(ethylene glycol) (PEG) in order to improve their specificity and bioactivity. PEG and the anti-MEL monoclonal antibody (mAb) were successfully immobilized on the surface of MNPs and characterized using FTIR, UV-Vis and TEM analyses. Surface modification of MNPs-PEG-mAb conjugates of a variety of sizes and magnetite types was employed to design and prepare labels for use in a lateral flow immunoassay (LFIA) to test whether the size of the conjugate can affect the performance of the assay. The results showed that the detection limit was mainly determined by the size of the MNPs-PEG-mAb conjugate. Under optimized conditions, a detection limit of 0.4 ppm for melamine was achieved using Fe2O3-PEG-mAb, which was almost 5-fold lower than that of the Fe3O4-PEG-mAb conjugate (2.2 ppm).
为了提高其特异性和生物活性，用聚乙二醇（PEG）对磁铁矿纳米颗粒（MNPs）Fe3O4和r-Fe2O3进行了表面改性。PEG和抗MEL单克隆抗体（mAb）成功固定在MNPs表面，并通过傅里叶变换红外光谱（FTIR）、紫外可见光谱（UV-Vis）和透射电子显微镜（TEM）分析进行了表征。采用对多种尺寸和磁铁矿类型的MNPs-PEG-mAb缀合物进行表面改性的方法，设计并制备了用于侧向流动免疫分析（LFIA）的标记物，以测试缀合物的尺寸是否会影响分析性能。结果表明，检测限主要由MNPs-PEG-mAb缀合物的尺寸决定。在优化条件下，使用Fe2O3-PEG-mAb对三聚氰胺的检测限达到0.4 ppm，几乎比Fe3O4-PEG-mAb缀合物（2.2 ppm）低5倍。
