Vigh G, Varga-Puchony Z, Szepesi G, Gazdag M
J Chromatogr. 1987 Jan 16;386:353-62. doi: 10.1016/s0021-9673(01)94612-6.
A reversed-phase displacement chromatographic method has been developed for the purification of bovine and porcine insulin samples. Up to 500 mg of raw insulin could be purified on a Nucleosil C8 analytical column using a methanol-containing phosphate buffer carrier and a cetrimide-containing displacer. A proinsulin contamination level as low as 100 ppm in the collected fractions could be achieved.
已开发出一种反相置换色谱法用于纯化牛胰岛素和猪胰岛素样品。使用含甲醇的磷酸盐缓冲载体和含十六烷基三甲基溴化铵的置换剂，在Nucleosil C8分析柱上可纯化高达500毫克的粗胰岛素。收集的馏分中胰岛素原污染水平可低至100 ppm。
