高效液相色谱法分离胰岛素及其衍生物中的协同相互作用。

Coordinative interactions in the separation of insulin and its derivatives by high-performance liquid chromatography.

作者信息

Péter A, Szepesi G, Baláspiri L, Burger K

机构信息

Department of Inorganic and Analytical Chemistry, A. József University, Szeged, Hungary.

出版信息

J Chromatogr. 1987 Nov 6;408:43-52. doi: 10.1016/s0021-9673(01)81789-1.

DOI:10.1016/s0021-9673(01)81789-1

PMID:3323216

Abstract

The effects of various inorganic cations and anions on the chromatographic behaviour of insulin and its derivatives have been investigated. It was shown that on an octadecylsilica column at a given eluent composition the retention of insulins depends primarily on the nature of the anions. The cations have a secondary effect, depending on their size and coordination ability. The mechanism of the effect of the ions is complex. It includes the formation of ion pairs, their influence on dissociation processes, a silanophilic effect, coordination processes and changes in the structures of the insulins.

摘要

研究了各种无机阳离子和阴离子对胰岛素及其衍生物色谱行为的影响。结果表明，在给定洗脱液组成的十八烷基硅胶柱上，胰岛素的保留主要取决于阴离子的性质。阳离子具有次要作用，这取决于它们的大小和配位能力。离子作用的机制很复杂。它包括离子对的形成、它们对解离过程的影响、硅醇基作用、配位过程以及胰岛素结构的变化。