Borja Karla Verónica, Amador Andrés Miguel, Parra Silvana Hipatia Santander, Cárdenas Cristian Fernando, Núñez Luis Fabian
Facultad de Ciencias de la Salud, Carrera de Medicina Veterinaria y Zootecnia; Universidad de Las Américas, Quito, Ecuador, Antigua Vía a Nayón S/N, Quito EC 170124.
Facultad de Ciencias de la Salud, Carrera de Medicina Veterinaria y Zootecnia, One Health Research Group; Universidad de Las Américas, Quito, Ecuador, Antigua Vía a Nayón S/N, Quito EC 170124.
Vet World. 2022 Mar;15(3):737-742. doi: 10.14202/vetworld.2022.737-742. Epub 2022 Mar 26.
Several Ecuadorian farms use human test strips (cheaper than veterinary strips) to diagnose bovine ketosis; however, their reliability is unknown. This study aimed to determine the confidence level of human strips for the detection of ketosis in bovines by comparing two diagnostic methods for ketosis: one used in bovines (gold standard) to analyze blood samples and the other used in humans to analyze urine samples.
The study was conducted on an Ecuadorian farm using 50 animals, ten from each of five categories: heifers, 4 months pregnant (4MP), 15 days prepartum (15DPRE), 15 days postpartum (15DPOST), and 42 days postpartum (42DPOST). Blood samples were collected through coccygeal venipuncture and urine samples were collected during spontaneous urination. BHBCheck™ assay was used to measure b-hydroxybutyrate (BHB) in the blood, whereas Combur10Test was used to measure acetoacetate (AcAc) in urine for the determination of ketosis.
BHB was detected in all animals. Based on a ketosis cutoff point of 0.8-1.2 mmol/L, 13 animals from the 15DPOST and 42DPOST categories had ketosis; AcAc was detected in the urine from nine animals originated from the two same categories. Metabolites, either BHB or AcAc, were not detected in heifers, 4MP, or 15DPRE individuals. Finally, the BHBCheck™ assay had better efficiency in detecting ketosis in animals (p<0.05) than the Combur10Test.
Combur10Test urine strips reached 92% reliability for the detection of ketosis in dairy cattle, compared to BHBCheck™ assays.
厄瓜多尔的一些养殖场使用人用检测试纸（比兽用试纸便宜）来诊断牛酮血症，但其可靠性未知。本研究旨在通过比较两种酮血症诊断方法来确定人用试纸检测牛酮血症的置信水平：一种是用于牛的（金标准）血液样本分析方法，另一种是用于人的尿液样本分析方法。
本研究在厄瓜多尔的一个养殖场进行，使用了50头动物，分为五个类别，每个类别10头：小母牛、怀孕4个月（4MP）、产前15天（15DPRE）、产后15天（15DPOST）和产后42天（42DPOST）。通过尾静脉穿刺采集血液样本，在自然排尿时采集尿液样本。使用BHBCheck™检测法测量血液中的β-羟基丁酸（BHB），而使用Combur10Test检测法测量尿液中的乙酰乙酸（AcAc）以确定酮血症。
在所有动物中均检测到BHB。基于0.8 - 1.2 mmol/L的酮血症临界值，15DPOST和42DPOST类别的13头动物患有酮血症；在来自相同两个类别的9头动物的尿液中检测到AcAc。在小母牛、4MP或15DPRE个体中未检测到代谢物BHB或AcAc。最后，BHBCheck™检测法在检测动物酮血症方面比Combur10Test具有更高的效率（p<0.05）。
与BHBCheck™检测法相比，Combur10Test尿试纸在检测奶牛酮血症方面的可靠性达到92%。
