Preparation and biological properties of silk fibroin/nano-hydroxyapatite/graphene oxide scaffolds with an oriented channel-like structure.

Constructing an ideal bone tissue engineering scaffold has been one of the research hotspots in the biomedical field. Silk fibroin (SF), nano-hydroxyapatite (nHAp) and graphene oxide (GO) are excellent biomaterials, and have been studied and explored extensively. To better mimic natural bone, we fabricated a SF/nHAp/GO hybrid scaffold with an oriented channel-like structure by using directional temperature field freezing technology. A comparative analysis was carried out for the SF, SF/nHAp, unoriented SF/nHAp/GO and oriented SF/nHAp/GO scaffolds. The physical and chemical properties were studied by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and universal mechanical testing. The data showed that the oriented channel-like SF/nHAp/GO porous scaffold expressed high interconnectivity, suitable pore diameter and porosity and anisotropic mechanical properties. Cytocompatibility tests indicated that the oriented channel-like SF/nHAp/GO porous scaffold was more favorable for stimulating bone marrow mesenchymal stem cells (BMSCs) adhesion and proliferation. Additionally, human umbilical vein endothelial cells (HUVECs) grew unimpeded along the channel, indicating it had advantages for vascularization. For further testing , osteogenic induction was carried out on BMSCs inoculated on the above scaffolds, and then alkaline phosphatase (ALP) activity was tested and cell mineralization was observed. The results indicated that the oriented channel-like SF/nHAp/GO porous scaffold was more conducive to osteogenic differentiation of BMSCs. Hence, the material may prove to be a promising scaffold for bone tissue engineering.