Lyu Zhongfan, Hao Yongchao, Chen Liyang, Xu Shoushen, Wang Hongjin, Li Mengyao, Ge Wenyang, Hou Bingqian, Cheng Xinxin, Li Xuefeng, Che Naixiu, Zhen Tianyue, Sun Silong, Bao Yinguang, Yang Zujun, Jia Jizeng, Kong Lingrang, Wang Hongwei
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, China.
Smartgenomics Technology Institute, Tianjin, China.
Front Plant Sci. 2022 Apr 15;13:837410. doi: 10.3389/fpls.2022.837410. eCollection 2022.
Even frequently used in wheat breeding, we still have an insufficient understanding of the biology of the products distant hybridization. In this study, a transcriptomic analysis was performed for six - substitution lines in comparison with the host plants. All the six disomic substitution lines showed much stronger "transcriptomic-shock" occurred on alien genomes with 57.43-69.22% genes changed expression level but less on the recipient genome (2.19-8.97%). Genome-wide suppression of alien genes along chromosomes was observed with a high proportion of downregulated genes (39.69-48.21%). Oppositely, the wheat recipient showed genome-wide compensation with more upregulated genes, occurring on all chromosomes but not limited to the homeologous groups. Moreover, strong co-upregulation of the orthologs between wheat and sub-genomes was enriched in photosynthesis with predicted chloroplastic localization, which indicates that the compensation happened not only on wheat host genomes but also on alien genomes.
即使在小麦育种中经常使用，我们对远缘杂交产物的生物学特性仍了解不足。在本研究中，对六个代换系与受体植株进行了转录组分析。所有六个二体代换系在外源基因组上均表现出更强的“转录组冲击”，57.43%-69.22%的基因表达水平发生变化，而在受体基因组上变化较小（2.19%-8.97%）。观察到沿着染色体对外源基因进行全基因组抑制，下调基因比例较高（39.69%-48.21%）。相反，小麦受体表现出全基因组补偿，上调基因更多，发生在所有染色体上，但不限于同源群。此外，小麦与亚基因组之间直系同源基因的强烈共上调在预测具有叶绿体定位的光合作用中富集，这表明补偿不仅发生在小麦受体基因组上，也发生在外源基因组上。
