Liu Qingxiu, Li Wenxiang, Zhang Jiatu, Zhao Lihong, Ji Cheng, Zhang Jianyun, Huang Shimeng, Ma Qiugang
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Vet Sci. 2022 Apr 4;9:875769. doi: 10.3389/fvets.2022.875769. eCollection 2022.
Fish oil (FO) is an important source of lipid in functional food and aquafeeds. However, the harmful effects of oxidized fish oil (OFO) on host metabolism and reproductive health are not yet clear. In addition, lipoamide (LAM) has been widely studied as an agent for alleviating various diseases associated with oxidative disruption. Therefore, in the current study, to investigate the effects of LAM in alleviating OFO-induced decline in reproductive performance and oxidative damage to the oviduct in laying hens. We constructed a 1% fresh FO model, a 1% OFO model, and a LAM model with 1% OFO (OFO + LAM) added at 100 mg/kg to explore the antioxidant effect of LAM. Herein, these results were evaluated by breeding performance, immune responses, estrogen, and antioxidant indices of serum samples, as well as the number of follicles and antioxidant parameters of oviducts. From the results, compared with the FO group, OFO significantly decreased the egg-laying rate, increased the contents of total protein (TP) and inflammatory factors [tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-8, and interferon γ (INF-γ)], and reduced the concentrations of anti-oxidation [total antioxidant (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), glutathione reductase (GR), catalase (CAT), and hydroxyl radical scavenging activity (HRSA)] in serum samples, as well as reduced the levels of anti-oxidation indexes in oviduct tissues ( < 0.05). Of note, the supplementation of LAM could significantly increase the laying performance, improve the levels of serum immunoglobulins (IgA, IgG, and IgM), serum estrogen [progesterone (P) and estradiol (E2)], and serum antioxidant parameters (T-AOC, T-SOD, GSH-Px, GSH, GR, CAT, and HRSA) and decrease the concentrations of serum inflammatory cytokines (TNF-α, IL-6, IL-8, and INF-γ) in laying hens following OFO administration ( < 0.05). In addition, LAM could dramatically increase the contents of antioxidant factors ( < 0.05) in oviducts and enhance the secretion capacity of the uterine part. Taken together, OFO caused host metabolic dysfunction, oxidative damage, uterine morphological abnormalities, and alterations of ovarian function. These results suggested that LAM administration could alleviate host metabolic dysfunctions and inflammatory damage, and then ameliorate oxidative damage in the oviduct induced by OFO, ultimately improving reproductive function.
鱼油（FO）是功能性食品和水产饲料中脂质的重要来源。然而，氧化鱼油（OFO）对宿主代谢和生殖健康的有害影响尚不清楚。此外，硫辛酰胺（LAM）作为一种缓解与氧化破坏相关的各种疾病的药物已得到广泛研究。因此，在本研究中，为了探究LAM对缓解OFO诱导的蛋鸡生殖性能下降和输卵管氧化损伤的作用。我们构建了1%新鲜FO模型、1%OFO模型以及添加了100mg/kg 1%OFO的LAM模型（OFO+LAM）来探究LAM的抗氧化作用。在此，通过繁殖性能、免疫反应、雌激素、血清样本的抗氧化指标以及卵泡数量和输卵管的抗氧化参数对这些结果进行评估。结果显示，与FO组相比，OFO显著降低了产蛋率，增加了总蛋白（TP）和炎症因子[肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-6、IL-8和干扰素γ（INF-γ）]的含量，并降低了血清样本中的抗氧化[总抗氧化能力（T-AOC）、总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽（GSH）、谷胱甘肽还原酶（GR）、过氧化氢酶（CAT）和羟自由基清除活性（HRSA）]浓度，以及降低了输卵管组织中的抗氧化指标水平（P<0.05）。值得注意的是，补充LAM可显著提高产蛋性能，改善血清免疫球蛋白（IgA、IgG和IgM）、血清雌激素[孕酮（P）和雌二醇（E2）]水平以及血清抗氧化参数（T-AOC、T-SOD、GSH-Px、GSH、GR、CAT和HRSA），并降低OFO给药后蛋鸡血清炎症细胞因子（TNF-α、IL-6、IL-8和INF-γ）的浓度（P<0.05）。此外，LAM可显著增加输卵管中抗氧化因子的含量（P<0.05）并增强子宫部分的分泌能力。综上所述，OFO导致宿主代谢功能障碍、氧化损伤、子宫形态异常和卵巢功能改变。这些结果表明，给予LAM可缓解宿主代谢功能障碍和炎症损伤，进而改善OFO诱导的输卵管氧化损伤，最终改善生殖功能。
