Remaggi Giulia, Saleri Roberta, Andrani Melania, Satolli Francesca, Rodighiero Eleonora, Elviri Lisa
Department of Food and Drug Science, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy.
Department of Veterinary Science, University of Parma, Via del Taglio 10, 43100 Parma, Italy.
Food Chem X. 2022 Feb 24;13:100271. doi: 10.1016/j.fochx.2022.100271. eCollection 2022 Mar 30.
A simple and reliable targeted liquid chromatography-electrospray-tandem mass spectrometry (LC-MS/MS) method was developed and validated through the selection of two biomarker peptides for the identification and determination of bovine insulin like growth factor-1 (IGF-1) in milk samples. Two urea-based sample extraction procedures were tested. The validation results provided detection limits at the 1-5 ng IGF-1/mL level as a function of the milk matrix, precision ranged from 3 to 8% and the method accuracy in the different milk matrices was assured. Finally, IGF-1 was measured in milk samples obtained by treatment with eleven different technological processes: IGF-1 concentrations were spread over a wide range from 11.2 ± 0.3 ng/mL to 346 ± 8 ng/mL with a median of 57.0 ± 0.2 ng/mL. The highest amount of IGF-1 was found in fresh whole milk samples and no significant correlation was found between the total milk protein content and the IGF-1 concentration level.
通过选择两种生物标志物肽，开发并验证了一种简单可靠的靶向液相色谱 - 电喷雾串联质谱（LC-MS/MS）方法，用于鉴定和测定牛奶样品中的牛胰岛素样生长因子 -1（IGF-1）。测试了两种基于尿素的样品提取程序。验证结果表明，根据牛奶基质的不同，检测限在1 - 5 ng IGF-1/mL水平，精密度范围为3%至8%，且该方法在不同牛奶基质中的准确性得到保证。最后，对通过十一种不同工艺处理获得的牛奶样品进行了IGF-1测定：IGF-1浓度分布范围很广，从11.2±0.3 ng/mL到346±8 ng/mL，中位数为57.0±0.2 ng/mL。在新鲜全脂牛奶样品中发现的IGF-1含量最高，且未发现总牛奶蛋白含量与IGF-1浓度水平之间存在显著相关性。
