Zhuang Shanshan, Bao Yao, Zhang Yaxin, Zhang Huangyou, Liu Jianliang, Liu Huifan
College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China.
Guangzhou Key Laboratory for Research and Development of Crop Germplasm Resources, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Food Chem X. 2022 Jan 29;13:100229. doi: 10.1016/j.fochx.2022.100229. eCollection 2022 Mar 30.
Previously, we found that ASP-ASP-ASP-TYR (DDDY) from has a minimum inhibitory concentration of 36.15 mg/mL against . Here, we explored the antibacterial mechanism of DDDY and its potential preservation applications. Metabolomic and transcriptomic analyses revealed that DDDY mainly affects genes involved in membrane transport and amino acid metabolism pathways. Molecular dynamics simulation revealed that DDDY had a stronger effect on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine phospholipid membranes than on 1-palmitoyl-2-oleoyl-lecithin or 1-palmitoyl-2-oleoyl phosphatidylglycerol membranes, with high DDDY concentrations displaying stronger efficacy on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine. Mechanistically, the -terminal of DDDY first bound to the phospholipid head group, while its C-terminal amino acid residue bound the hydrophobic tail, thereby creating a gap in the membrane when the phospholipids were clustered by hydrogen bonding. Finally, DDDY inhibited the growth of food microorganisms inoculated onto chestnut kernels, suggesting that DDDY is a promising antibacterial agent against multidrug-resistant gram-negative bacteria.
此前，我们发现来自[具体来源未提及]的ASP-ASP-ASP-TYR（DDDY）对[具体菌种未提及]的最低抑菌浓度为36.15毫克/毫升。在此，我们探究了DDDY的抗菌机制及其潜在的保鲜应用。代谢组学和转录组学分析表明，DDDY主要影响参与膜转运和氨基酸代谢途径的基因。分子动力学模拟显示，DDDY对1-棕榈酰-2-油酰-sn-甘油-3-磷酸乙醇胺磷脂膜的影响比对1-棕榈酰-2-油酰卵磷脂或1-棕榈酰-2-油酰磷脂酰甘油膜的影响更强，高浓度的DDDY对1-棕榈酰-2-油酰-sn-甘油-3-磷酸乙醇胺显示出更强的功效。从机制上讲，DDDY的N端首先与磷脂头部基团结合，而其C端氨基酸残基与疏水尾部结合，从而在磷脂通过氢键聚集时在膜上形成一个间隙。最后，DDDY抑制了接种在板栗仁上的食品微生物的生长，这表明DDDY是一种有前景的抗多重耐药革兰氏阴性菌的抗菌剂。
