Bennett J E, Friedman M M, Dupont B
J Infect Dis. 1987 May;155(5):1005-10. doi: 10.1093/infdis/155.5.1005.
The fate of radiolabeled Aspergillus fumigatus galactomannan was studied after intravenous injection into rabbits and rats. At 1 hr, the liver contained 35% of the injected dose in rabbits and 30% in rats. Excretion of galactomannan into the urine, measured in rabbits, was another major catabolic route and accounted for 35% of the dose by 24 hr. Immunization of rabbits increased hepatic uptake and decreased urinary excretion. Hepatic uptake in unimmunized rats could be decreased by Saccharomyces cerevisiae mannan, alpha-methyl mannoside, and N-acetylglucosamine, known inhibitors of the macrophage mannose receptor. Autoradiography showed hepatic radiolabeled galactomannan to be concentrated in Kupffer cells, which express the mannosyl receptor for glycoproteins. Macrophage mannosyl receptors may constitute a general mechanism for clearing fungal mannans from the bloodstream.
将放射性标记的烟曲霉半乳甘露聚糖静脉注射到兔子和大鼠体内后，对其命运进行了研究。1小时时，肝脏在兔子体内含35%的注射剂量，在大鼠体内含30%。在兔子中测定的半乳甘露聚糖向尿液中的排泄是另一条主要的分解代谢途径，到24小时时占剂量的35%。兔子免疫后肝脏摄取增加，尿液排泄减少。酿酒酵母甘露聚糖、α-甲基甘露糖苷和N-乙酰葡糖胺（已知的巨噬细胞甘露糖受体抑制剂）可降低未免疫大鼠的肝脏摄取。放射自显影显示肝脏放射性标记的半乳甘露聚糖集中在库普弗细胞中，库普弗细胞表达糖蛋白的甘露糖基受体。巨噬细胞甘露糖基受体可能是从血液中清除真菌甘露聚糖的一般机制。
