School of Food Science and Technology, Dalian Polytechnic University, No. 1 Qinggongyuan, Ganjingzi District, Liaoning, 116034, People's Republic of China.
Probiotics Antimicrob Proteins. 2022 Aug;14(4):741-750. doi: 10.1007/s12602-022-09917-5. Epub 2022 May 2.
The aim of the present study was to investigate the in vitro antioxidant potential of Bacillus coagulans T242. B. coagulans T242 showed better antioxidant activities, including the 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging ability, lipid peroxidation inhibiting ability and reducing ability, than those exerted by Lactobacillus rhamnosus GG (LGG). B. coagulans T242 positively regulated the expression of the nuclear factor erythroid 2-relatedfactor 2/Kelch-like ECH-associated protein-1 (Nrf2/Keap1) pathway-related proteins (Nrf2, Keap1, heine oxygenase-1 (HO-1)); increased antioxidant enzymes (glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD)); reduced the content of malondialdehyde (MDA) level; decreased the expression of inflammatory-related cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α); and thus increased the survival rate in 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH)-damaged HT-29 cells. This study proved that B. coagulans T242 exerted antioxidative effects by quenching oxygen free radicals and activating the Nrf2 signaling pathway in HT-29 cells.
本研究旨在探究凝结芽孢杆菌 T242 的体外抗氧化潜力。与鼠李糖乳杆菌 GG（LGG）相比，凝结芽孢杆菌 T242 表现出更好的抗氧化活性，包括 2,2'- 连氮 - 双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力、抑制脂质过氧化能力和还原能力。凝结芽孢杆菌 T242 可正向调节核因子红细胞 2 相关因子 2/kelch 样 ECH 相关蛋白 1（Nrf2/Keap1）通路相关蛋白（Nrf2、Keap1、血红素加氧酶-1（HO-1））的表达；增加抗氧化酶（谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD））的含量；降低丙二醛（MDA）水平；降低炎症相关细胞因子白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的表达；从而提高 2,2'-偶氮双（2-甲基丙脒）二盐酸盐（AAPH）损伤的 HT-29 细胞的存活率。本研究证明，凝结芽孢杆菌 T242 通过清除氧自由基和激活 HT-29 细胞中的 Nrf2 信号通路发挥抗氧化作用。
