Iloba Ifeanyi, McGarry Sage V, Yu Liu, Cruickshank Dina, Jensen Gitte S
NIS Labs, 1437 Esplanade, Klamath Falls, OR 97601, USA.
NIS Labs, 807 St. George St., Port Dover, ON N0A 1N0, Canada.
Microorganisms. 2023 Oct 15;11(10):2564. doi: 10.3390/microorganisms11102564.
Spore-forming probiotic bacteria, including , are resilient and produce a variety of beneficial metabolites. We evaluated the immune-modulating effects of the novel probiotic strain JBI-YZ6.3, where the germinated spores, metabolite fraction, and cell wall fraction were tested in parallel using human peripheral blood mononuclear cell cultures under both normal and lipopolysaccharide-induced inflamed culture conditions. The expression of CD25 and CD69 activation markers was evaluated via flow cytometry. Supernatants were tested for cytokines, interferons, chemokines, and growth factors using Luminex arrays. The germinated spores were highly immunogenic; both the cell wall and metabolite fractions contributed significantly. Under normal culture conditions, increased levels of immune activation were observed as increased expressions of CD25 and CD69 relative to natural killer cells, suggesting an increased ability to attack virus-infected target cells. On monocytes, a complex effect was observed, where the expression of CD25 increased under normal conditions but decreased under inflamed conditions. This, in combination with increased interleukin-10 (IL-10) and decreased monocyte chemoattractant protein-1 (MCP-1) production under inflamed conditions, points to anti-inflammatory effects. The production of the stem cell-related growth factor granulocyte colony-stimulating Factor (G-CSF) was enhanced. Further research is warranted to characterize the composition of the postbiotic metabolite fraction and document the characteristics of immunomodulating agents secreted by this probiotic strain.
包括芽孢杆菌在内的产孢益生菌具有很强的适应能力，并能产生多种有益代谢产物。我们评估了新型益生菌菌株JBI-YZ6.3的免疫调节作用，在正常和脂多糖诱导的炎症培养条件下，使用人外周血单核细胞培养物对其萌发的孢子、代谢产物组分和细胞壁组分进行了平行测试。通过流式细胞术评估CD25和CD69激活标志物的表达。使用Luminex阵列检测上清液中的细胞因子、干扰素、趋化因子和生长因子。萌发的孢子具有高度免疫原性；细胞壁和代谢产物组分均有显著贡献。在正常培养条件下，相对于自然杀伤细胞，CD25和CD69表达增加，表明攻击病毒感染靶细胞的能力增强，免疫激活水平升高。在单核细胞上观察到复杂的效应，正常条件下CD25表达增加，但在炎症条件下降低。这与炎症条件下白细胞介素-10（IL-10）产生增加和单核细胞趋化蛋白-1（MCP-1）产生减少相结合，表明具有抗炎作用。干细胞相关生长因子粒细胞集落刺激因子（G-CSF）的产生增强。有必要进一步研究以表征后生元代谢产物组分的组成，并记录该益生菌菌株分泌的免疫调节剂的特性。
