构建并鉴定重组型 CRF63_02A 和 A 亚型 HIV-1 包膜假病毒
Construction and Characterization of HIV-1 env-Pseudoviruses of the Recombinant Form CRF63_02A and Subtype A6.
机构信息
State Research Center of Virology and Biotechnology Vector, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.
出版信息
Bull Exp Biol Med. 2022 Apr;172(6):729-733. doi: 10.1007/s10517-022-05466-7. Epub 2022 May 2.
HIV-1 env-pseudoviruses are a useful tool in the search for antiviral drugs (entry inhibitors) and evaluation of the efficacy of HIV-1 vaccines. Given the high genetic variability of HIV-1, it is necessary to regularly update the panels of pseudoviruses in accordance with the emergence of new strains. Based on genetic variants of HIV-1 circulating in the regions of the Siberian Federal District, 13 HIV-1 env-pseudoviruses of recombinant form CRF63_02A and subtype A6 were obtained. Most pseudoviruses have been shown to be sensitive to neutralization by bnAbs VRC01, PGT126, and 10E8, moderately sensitive to bnAbs PG9 and 4E10, and resistant to bnAbs 2G12, PG16, and 2F5. All obtained variants of pseudoviruses are CCR5-tropic.
HIV-1 env 假病毒是寻找抗病毒药物（进入抑制剂）和评估 HIV-1 疫苗功效的有用工具。鉴于 HIV-1 的高度遗传变异性，有必要根据新菌株的出现定期更新假病毒的面板。基于在西伯利亚联邦区流行的 HIV-1 遗传变异，获得了 13 种重组形式 CRF63_02A 和亚型 A6 的 HIV-1 env 假病毒。大多数假病毒已被证明对 bnAbs VRC01、PGT126 和 10E8 的中和作用敏感，对 bnAbs PG9 和 4E10 中度敏感，对 bnAbs 2G12、PG16 和 2F5 具有抗性。所有获得的假病毒变体均为 CCR5 嗜性。
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