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一种新型卵巢高级别浆液性癌细胞系IPO43的建立与鉴定

Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line-IPO43.

作者信息

Silva Fernanda, Coelho Filipa, Peixoto Ana, Pinto Pedro, Martins Carmo, Frombach Ann-Sophie, Santo Vítor E, Brito Catarina, Guimarães António, Félix Ana

机构信息

Chronic Diseases Research Center, (CEDOC-FCM-UNL), NOVA Medical School, NMS, Universidade NOVA de Lisboa, 1169-056, Lisbon, Portugal.

Molecular Pathobiology Research Unit, Portuguese Institute of Oncology Francisco Gentil Lisbon (IPOLFG), 1099-023, Lisbon, Portugal.

出版信息

Cancer Cell Int. 2022 Apr 30;22(1):175. doi: 10.1186/s12935-022-02600-3.

DOI:10.1186/s12935-022-02600-3
PMID:35501869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9063187/
Abstract

BACKGROUND

Epithelial ovarian cancer (EOC) is an aggressive and lethal malignancy and novel EOC cell lines with detailed characterization are needed, to provide researchers with diverse helpful resources to study EOC biological processes and cancer experimental therapies.

METHODS

The IPO43 cell line was established from the ascitic fluid of a patient with a diagnosis of high-grade serous carcinoma (HGSC) of the ovary, previously treated with chemotherapy. Cell immortalization was achieved in 2D cell culture and growth obtained in 2D and 3D cell cultures. The characterization of immortalized cells was done by immunocytochemistry, flow cytometry, cell proliferation, chromosomal Comparative Genomic Hybridization (cCGH), STR profile and Next Generation Sequencing (NGS).

RESULTS

Characterization studies confirmed that IPO43 cell line is of EOC origin and maintains morphological and molecular features of the primary tumor. cCGH analysis showed a complex profile with gains and losses of specific DNA regions in both primary ascitic fluid and cell line IPO43. The cell line was successfully grown in a 3D system which allows its future application in more complex assays than those performed in 2D models. IPO43 cell line is resistant to standard drug treatment in vitro.

CONCLUSIONS

IPO43 is available for public research and we hope it can contribute to enrich the in vitro models addressing EOC heterogeneity, being useful to investigate EOC and to develop new therapeutic modalities.

摘要

背景

上皮性卵巢癌（EOC）是一种侵袭性和致死性恶性肿瘤，需要具有详细特征描述的新型EOC细胞系，为研究人员提供多样化的有用资源，以研究EOC生物学过程和癌症实验疗法。

方法

IPO43细胞系是从一名诊断为卵巢高级别浆液性癌（HGSC）且先前接受过化疗的患者的腹水中建立的。在二维细胞培养中实现细胞永生化，并在二维和三维细胞培养中获得生长。通过免疫细胞化学、流式细胞术、细胞增殖、染色体比较基因组杂交（cCGH）、STR图谱和下一代测序（NGS）对永生化细胞进行特征描述。

结果

特征研究证实IPO43细胞系起源于EOC，并保留了原发肿瘤的形态和分子特征。cCGH分析显示，原发腹水和IPO43细胞系中特定DNA区域均存在复杂的增减图谱。该细胞系在三维系统中成功生长，这使其未来可应用于比二维模型更复杂的检测。IPO43细胞系在体外对标准药物治疗具有抗性。

结论

IPO43可供公众研究使用，我们希望它能有助于丰富针对EOC异质性的体外模型，对研究EOC和开发新的治疗方式有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/54681050db73/12935_2022_2600_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/0808d2497753/12935_2022_2600_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/30f26d322371/12935_2022_2600_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/1490072fa761/12935_2022_2600_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/9a49dd70ccf4/12935_2022_2600_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/50b980e99fb1/12935_2022_2600_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/cf40ab610133/12935_2022_2600_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/5cd1e05d284f/12935_2022_2600_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/54681050db73/12935_2022_2600_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/0808d2497753/12935_2022_2600_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/5c4fe1c2bffd/12935_2022_2600_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/30f26d322371/12935_2022_2600_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/1490072fa761/12935_2022_2600_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/9a49dd70ccf4/12935_2022_2600_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/50b980e99fb1/12935_2022_2600_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/cf40ab610133/12935_2022_2600_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/5cd1e05d284f/12935_2022_2600_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6fb/9063187/54681050db73/12935_2022_2600_Fig9_HTML.jpg

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