渗透压变化对人正常髓核间充质干细胞生物学活性的影响

Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells.

作者信息

Zhang Hua, Li Wei, Wu YaoHong, Zhang Shengquan, Li Jie, Han Letian, Chen Haoyu, Wang Ziyu, Shen Cailiang, Zhang Yinshun, Tao Hui

机构信息

Department of Orthopedics & Spine Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022 Anhui, China.

Department of Orthopedics, Huaibei People's Hospital, Huaibei, 235000 Anhui, China.

出版信息

Stem Cells Int. 2022 Apr 23;2022:1121064. doi: 10.1155/2022/1121064. eCollection 2022.

DOI:10.1155/2022/1121064

PMID:35502327

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9056247/

Abstract

The expansion and maintenance of the NPMSC (nucleus pulposus mesenchymal stem cell) phenotype are considered as potential therapeutic tools for clinical applications in intervertebral disc tissue engineering and regenerative medicine. However, the harsh microenvironment within the intervertebral disc is the main limitation of its regeneration. The osmolarity of the intervertebral disc is higher than that of other tissues, which has an important influence on the biological characteristics of NPMSCs. In this study, we observed the effect of different osmolarities on the biological characteristics of human normal NPMSCs cultured in vitro and explored the role of osmolarity in intervertebral disc degeneration. Our data demonstrated that the change in osmotic pressure has an important effect on the biological activity of NPMSCs, and this effect may occur through the P16/Rb pathway. This study provides a theoretical basis for the future treatment of intervertebral disc degeneration.

摘要

髓核间充质干细胞（NPMSC）表型的扩展和维持被视为椎间盘组织工程和再生医学临床应用的潜在治疗工具。然而，椎间盘内恶劣的微环境是其再生的主要限制因素。椎间盘的渗透压高于其他组织，这对NPMSCs的生物学特性有重要影响。在本研究中，我们观察了不同渗透压对体外培养的人正常NPMSCs生物学特性的影响，并探讨了渗透压在椎间盘退变中的作用。我们的数据表明，渗透压的变化对NPMSCs的生物学活性有重要影响，且这种影响可能通过P16/Rb途径发生。本研究为未来治疗椎间盘退变提供了理论依据。