State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016, Shenyang, China.
Chem Biodivers. 2022 Jun;19(6):e202200286. doi: 10.1002/cbdv.202200286. Epub 2022 May 17.
Longibrachiamide A (1), a new 20-residue peptaibol, along with three known ones (2-4), were isolated from the fungus Trichoderma longibrachiatum Rifai DMG-3-1-1, isolated from a mushroom Clitocybe nebularis (Batsch) P. Kumm, which was collected from coniferous forest of northeast China in our previous work. The structure of longibrachiamide A (1) was determined by its NMR and ESI-MS/MS data, the absolute configuration of 1 was further determined by Marfey's analyses. And the complete NMR data of 2-4 were also reported for the first time. The similar CD spectra of 1-4 showed that they all had mixed 3 -/α-helical conformations. Compounds 1-4 showed strong cytotoxicities against BV2, A549 and MCF-7 cells, and also showed moderate inhibitory effects against the tested Gram-positive bacteria, including MRSA T144 and VRE-10.
从中国东北的针叶林中采集的蘑菇 Clitocybe nebularis (Batsch) P. Kumm 中分离出的真菌 Trichoderma longibrachiatum Rifai DMG-3-1-1，我们之前的工作中分离得到了一种新的 20 残基肽长菌素 Longibrachiamide A（1），以及三种已知的肽长菌素（2-4）。通过其 NMR 和 ESI-MS/MS 数据确定了 Longibrachiamide A（1）的结构，通过 Marfey 分析进一步确定了 1 的绝对构型。同时，我们还首次报道了 2-4 的完整 NMR 数据。1-4 的相似 CD 谱表明它们都具有混合的 3 -/α-螺旋构象。化合物 1-4 对 BV2、A549 和 MCF-7 细胞表现出强烈的细胞毒性，对测试的革兰氏阳性菌，包括 MRSA T144 和 VRE-10，也表现出中等抑制作用。
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