Setargie Adigo, Wang Chen, Zhang Liwen, Xu Yuquan
Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
Institute of Biotechnology, Bahir Dar University, P.O. Box. 79, Bahir Dar, Ethiopia.
Eng Microbiol. 2023 Dec 16;4(2):100135. doi: 10.1016/j.engmic.2023.100135. eCollection 2024 Jun.
Peptaibiotics are linear or cyclic peptide antibiotics characterized by the non-proteinogenic amino acid, alpha-aminoisobutyric acid. They exhibit a wide range of bioactivity against various pathogens. This report presents a comprehensive review of analytical methods for cultivation, production, isolation, screening, purification, and characterization of peptaibiotics, along with their bioactivity. Numerous techniques are currently available for each step, and we focus on describing the most commonly used and recently developed chromatographic and spectroscopic techniques. Investigating peptaibiotics requires efficient culture media, growth conditions, and isolation and purification techniques. The combination of chromatographic and spectroscopic tools offers a better opportunity for characterizing and identifying peptaibiotics. The evaluation of the chemical and biological properties of this compound has also been explored concerning its potential application in pharmaceutical and other industries. This review aims to summarize available data on the techniques and tools used to screen and purify peptaibiotics from fungi and bioactivity against various pathogens.
短杆菌肽类是一类线性或环状肽抗生素，其特征在于含有非蛋白质氨基酸α-氨基异丁酸。它们对多种病原体具有广泛的生物活性。本报告全面综述了短杆菌肽类的培养、生产、分离、筛选、纯化和表征的分析方法，以及它们的生物活性。目前每个步骤都有许多技术可用，我们重点描述最常用和最近开发的色谱和光谱技术。研究短杆菌肽类需要高效的培养基、生长条件以及分离和纯化技术。色谱和光谱工具的结合为表征和鉴定短杆菌肽类提供了更好的机会。还探讨了该化合物的化学和生物学性质评估及其在制药和其他行业的潜在应用。本综述旨在总结从真菌中筛选和纯化短杆菌肽类的技术和工具以及其对各种病原体生物活性的现有数据。
