Institute of Legal Medicine, University Hospital Bonn, Stiftsplatz 12, 53111 Bonn, Germany.
Institute of Legal Medicine, University Hospital Bonn, Stiftsplatz 12, 53111 Bonn, Germany.
Forensic Sci Int. 2022 Jul;336:111316. doi: 10.1016/j.forsciint.2022.111316. Epub 2022 Apr 25.
The expression levels of intracellular heat shock proteins (Hsps), specialized chaperone proteins, increase in cases of cellular stress with protein misfolding and aggregation. In a previous study, we demonstrated that there is an extensive increase in intracellular Hsp27 and 70 expression levels in renal tissues in fire fatality cases. Hsp expression can be induced by not only heat, but also by tissue hypoxia. In cases of fatal hemorrhage, the individual suffers hypoxemia and consequently tissue hypoxia. Here, we examined 43 cases of fatal hemorrhage and a control group of 85 deaths not related to blood loss or temperature exposure. We evaluated Hsp27 and 70 protein expression levels in renal tissue using immunohistochemistry. The results revealed that no extensive Hsp27 or 70 expression is induced in the fatal hemorrhage cases. The renal Hsp levels were similar to those of the control group. Fatal blood loss does not cause relevant cell stress.
细胞内热休克蛋白（Hsps）的表达水平在蛋白质错误折叠和聚集导致细胞应激时会增加。在之前的一项研究中，我们证明在火灾死亡病例的肾脏组织中，细胞内 Hsp27 和 70 的表达水平广泛增加。Hsp 的表达不仅可以由热诱导，还可以由组织缺氧诱导。在致命性出血的情况下，个体会出现低氧血症，进而导致组织缺氧。在这里，我们检查了 43 例致命性出血病例和 85 例与失血或温度暴露无关的对照组死亡病例。我们使用免疫组织化学评估了肾组织中 Hsp27 和 70 蛋白的表达水平。结果表明，在致命性出血病例中没有诱导广泛的 Hsp27 或 70 表达。肾组织中的 Hsp 水平与对照组相似。致命性失血不会导致相关的细胞应激。
