Doberentz E, Genneper L, Böker D, Lignitz E, Madea B
Institute of Legal Medicine, University of Bonn, Stiftsplatz 12, 53111, Bonn, Germany,
Int J Legal Med. 2014 Nov;128(6):967-78. doi: 10.1007/s00414-014-0994-0. Epub 2014 Apr 17.
The expression of heat shock proteins (hsp) increases in case of variable types of endogenous and exogenous cellular stress, as for example thermal stress. Immunohistochemical staining with hsp antibodies can visualize these stress proteins. Fifty-three cases of death due to heat and a control group of 100 deaths without any antemortem thermic stress were examined regarding hsp27 and hsp70 expression in myocardial, pulmonary, and renal tissues. The results revealed a correlation between hsp expression, survival time, and cause of death. In cases of death due to fire, the expression of hsp is more extensive than in the control group, especially in pulmonary and renal tissues. The immunohistochemical investigation of an hsp expression can support the proof of vitality in cases of death related to fire.
在各种内源性和外源性细胞应激情况下，如热应激，热休克蛋白（hsp）的表达会增加。用hsp抗体进行免疫组织化学染色可使这些应激蛋白可视化。对53例因热致死的病例以及100例生前无任何热应激的死亡对照组，就心肌、肺和肾组织中hsp27和hsp70的表达进行了检查。结果显示hsp表达、存活时间和死因之间存在相关性。在因火灾致死的病例中，hsp的表达比对照组更广泛，尤其是在肺和肾组织中。对hsp表达进行免疫组织化学研究可辅助证明与火灾相关死亡病例中的生前生命体征。
