Institute for Nanotechnology Innovation, Rhodes University, Makhanda 6140, South Africa.
Institute for Nanotechnology Innovation, Rhodes University, Makhanda 6140, South Africa.
Bioelectrochemistry. 2022 Aug;146:108146. doi: 10.1016/j.bioelechem.2022.108146. Epub 2022 Apr 29.
The role of the biointerface design towards the development of an impedimetric biosensor for the electrochemical detection of human epidermal growth factor receptor 2 (HER2) is investigated. Two novel cobalt phthalocyanines: cobalt tetraphenyl acetic acid phthalocyanine and cobalt tetraphenyl propionic acid phthalocyanine are compared as signal amplifiers and immobilization platforms of the HB5 aptamer towards the electrochemical detection of HER2. In addition, the phthalocyanines are coupled with the metal based cerium oxide nanoparticles. The efficiency of each electrode modification step and the performance of the constructed aptasensors were assessed by impedance spectroscopy. The aptasensors showed very low limit of detection values (all less than 0.2 ng/mL) with high sensitivity and stability. Furthermore, the aptasensors showed very good performance even in human serum samples. Considering these results, the aptasensors demonstrate great potential for improved monitoring of human epidermal growth factor receptor 2 levels for the management of breast cancers.
研究了生物界面设计在电化学生物传感器发展中的作用，该传感器用于电化学检测人表皮生长因子受体 2（HER2）。两种新型钴酞菁：四苯基乙酸钴酞菁和四苯基丙酸钴酞菁被用作 HB5 适体的信号放大器和固定化平台，用于电化学生物检测 HER2。此外，酞菁与基于金属的氧化铈纳米粒子结合。通过阻抗谱评估了每个电极修饰步骤的效率和构建的适体传感器的性能。该适体传感器的检测限非常低（均小于 0.2ng/mL），具有高灵敏度和稳定性。此外，该适体传感器在人血清样本中也表现出非常好的性能。考虑到这些结果，该适体传感器在提高人表皮生长因子受体 2 水平监测方面具有很大的潜力，可用于乳腺癌的管理。
