Department of Electronic & Electrical Engineering, University of Bath, Bath BA2 7AY, United Kingdom.
Department of Electronic & Electrical Engineering, University of Bath, Bath BA2 7AY, United Kingdom.
Biosens Bioelectron. 2018 Apr 15;102:106-112. doi: 10.1016/j.bios.2017.11.013. Epub 2017 Nov 8.
We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker. The sensor was characterized using cyclic voltammetry, electrochemical impedance spectroscopy (EIS), atomic force microscopy and contact angle studies. Non-Faradic EIS measurements were utilized to investigate the sensor performance via monitoring of the changes in capacitance. The aptasensor exhibited logarithmically detection of HER2 from 1pM to 100nM in both buffer and undiluted serum with limits of detection lower than 1pM. The results pave the way to develop other aptamer-based biosensors for protein biomarkers detection in undiluted serum.
我们报告了一种简单而强大的电容适体传感器的开发，用于检测和估计人表皮生长因子受体 2（HER2），这是乳腺癌的生物标志物，在未稀释的血清中。该研究涉及到叉指金电极的结合，这些电极被用来制备电化学平台。一种硫醇末端的 DNA 适体与 HER2 具有亲和力，通过自组装在叉指金表面上制备生物识别层。通过起始块磷酸盐缓冲盐水-Tween20 阻滞剂在表面上阻止非特异性结合，从而阻止非特异性结合。通过监测电容的变化，利用循环伏安法、电化学阻抗谱（EIS）、原子力显微镜和接触角研究对传感器进行了表征。非 Faradic EIS 测量被用于通过监测电容的变化来研究传感器的性能。该适体传感器在缓冲液和未稀释的血清中对 HER2 进行了对数检测，检测范围从 1pM 到 100nM，检测限低于 1pM。研究结果为开发其他基于适体的生物传感器用于未稀释血清中蛋白质生物标志物的检测铺平了道路。
