Institute for Nanotechnology Innovation, Chemistry Department, Rhodes University, Grahamstown 6140, South Africa.
Institute for Nanotechnology Innovation, Chemistry Department, Rhodes University, Grahamstown 6140, South Africa.
J Inorg Biochem. 2021 Aug;221:111462. doi: 10.1016/j.jinorgbio.2021.111462. Epub 2021 Apr 15.
Herein, a novel aptasensor is developed for the electrochemical detection of prostate specific antigen (PSA) on electrode surfaces modified using various combinations of a Cobalt phthalocyanine (CoPc), an aptamer and graphene quantum dots (GQDs). Electrochemical impedance spectroscopy (EIS) as well as differential pulse voltammetry (DPV) are employed for the detection of PSA. In both analytical techniques, linear calibration curves were observed at a concentration range of 1.2-2.0 pM. The glassy carbon electrode where CoPc and GQDs are placed on the electrode when non-covalently linked followed by addition of the aptamer (GQDs-CoPc(ππ)-aptamer (sequential)) showed the best performance with a limit of detection (LoD) as low as 0.66 pM when using DPV. The detection limits were much lower than the dangerous levels reported for PSA in males tested for prostate cancer. This electrode showed selectivity for PSA in the presence of bovine serum albumin, glucose and L-cysteine. The aptasensor showed good stability, reproducibility and repeatability, deeming it a promising early detection device for prostate cancer.
本文开发了一种新型适体传感器，用于在经各种组合的钴酞菁（CoPc）、适体和石墨烯量子点（GQDs）修饰的电极表面上进行电化学检测前列腺特异性抗原（PSA）。电化学阻抗谱（EIS）和差分脉冲伏安法（DPV）用于检测 PSA。在这两种分析技术中，在 1.2-2.0 pM 的浓度范围内观察到线性校准曲线。当非共价连接时，将 CoPc 和 GQDs 放置在电极上的玻碳电极，然后加入适体（GQDs-CoPc(ππ)-aptamer（顺序））时，使用 DPV 检测到的检测限（LoD）低至 0.66 pM，性能最佳。检测限远低于前列腺癌男性检测报告的 PSA 危险水平。该电极在存在牛血清白蛋白、葡萄糖和 L-半胱氨酸的情况下对 PSA 表现出选择性。该适体传感器表现出良好的稳定性、重现性和可重复性，有望成为前列腺癌的早期检测设备。
