Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, USA; IBBM, FCE-UNLP CONICET, La Plata 1900, Argentina.
Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, USA.
Dev Cell. 2022 May 9;57(9):1177-1192.e6. doi: 10.1016/j.devcel.2022.04.013. Epub 2022 May 2.
Understanding how roots modulate development under varied irrigation or rainfall is crucial for development of climate-resilient crops. We established a toolbox of tagged rice lines to profile translating mRNAs and chromatin accessibility within specific cell populations. We used these to study roots in a range of environments: plates in the lab, controlled greenhouse stress and recovery conditions, and outdoors in a paddy. Integration of chromatin and mRNA data resolves regulatory networks of the following: cycle genes in proliferating cells that attenuate DNA synthesis under submergence; genes involved in auxin signaling, the circadian clock, and small RNA regulation in ground tissue; and suberin biosynthesis, iron transporters, and nitrogen assimilation in endodermal/exodermal cells modulated with water availability. By applying a systems approach, we identify known and candidate driver transcription factors of water-deficit responses and xylem development plasticity. Collectively, this resource will facilitate genetic improvements in root systems for optimal climate resilience.
了解根在不同灌溉或降雨条件下如何调节发育对于培育具有气候适应能力的作物至关重要。我们建立了一套标记水稻品系工具，用于在特定细胞群体中对翻译 mRNA 和染色质可及性进行分析。我们利用这些工具在多种环境下研究了根：实验室培养皿、温室受控胁迫和恢复条件以及水田户外环境。整合染色质和 mRNA 数据可以解析以下调控网络：增殖细胞中的周期基因在淹没条件下减弱 DNA 合成；参与生长素信号转导、生物钟和地上组织小 RNA 调控的基因；以及内皮层/外皮层细胞中与水分可用性相关的木质素生物合成、铁转运蛋白和氮同化。通过应用系统方法，我们确定了已知和候选的与水分亏缺响应和木质部发育可塑性相关的驱动转录因子。总的来说，这个资源将有助于优化根系统对气候的适应能力的遗传改良。
