School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan.
Biochim Biophys Acta Biomembr. 2022 Sep 1;1864(9):183933. doi: 10.1016/j.bbamem.2022.183933. Epub 2022 Apr 30.
For analysis of the structure of human skin stratum corneum (SC), we introduced low-flux electron diffraction (ED) and developed a new statistical analysis method for obtained ED intensity profiles. By use of this method we compared the differences in the intercellular lipid organization on the SC corneocytes collected at human forehead, cheek, and forearm by the grid-stripping method. As a result, we found a significant regional difference in the distribution of lipid hydrocarbon chain packing domains in the SC; the ring-type ED pattern with orthorhombic symmetry was more often observed in the forearm SC than in the forehead and cheek SCs. We also found that the dependence of the background electron diffraction intensity on the modulus of the scattering vector differed significantly among them. The present method for the analysis of a large number of ED patterns of noninvasively obtained SC samples could be a powerful tool to scrutinize the structural difference between the SCs under various experimental conditions.
为了分析人类皮肤角质层（SC）的结构，我们引入了低通量电子衍射（ED），并开发了一种新的统计分析方法来处理得到的 ED 强度曲线。通过该方法，我们比较了用网格剥离法从人类前额、脸颊和前臂采集的 SC 角质细胞之间细胞间脂质组织的差异。结果发现，SC 中脂质烃链堆积区域的分布存在显著的区域差异；在 SC 中，具有正交对称性的环形 ED 模式在前臂 SC 中比在前额和脸颊 SC 中更为常见。我们还发现，背景电子衍射强度对散射矢量模量的依赖性在它们之间有很大的差异。本研究中对大量非侵入性获得的 SC 样本 ED 图谱进行分析的方法可能是一种强大的工具，可以仔细研究不同实验条件下 SC 之间的结构差异。
