Aldahlawi Salwa, Youssef Abdel-Rahman, Shahabuddin Syed
Department of Basic and Clinical Oral Sciences, College of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia,
Abbvie, North Chicago, IL, USA.
J Inflamm Res. 2018 Oct 31;11:389-396. doi: 10.2147/JIR.S177188. eCollection 2018.
The aim of this study was to evaluate CXCL10 as a biomarker for periodontitis by determining the CXCL10 levels in saliva, serum, and gingival crevicular fluid (GCF) samples from periodontally healthy control subjects and adult subjects with chronic periodontitis.
Adult patients seeking dental treatment at Umm Al-Qura University dental clinic underwent a complete periodontal examination, and saliva, serum, and GCF samples were collected. Subjects were classified as chronic periodontitis patients (n=31) if they have a periodontal probing depth (PD) of ≥4 mm and/or clinical attachment level (CAL) of ≥3 mm in >30% of the teeth. The control group (n=25) had PD ≤3 mm and/or CAL ≤2 mm. ELISA was performed to determine the concentration of CXCL10 in saliva, serum, and GCF samples. Student's -test was carried out to evaluate the significant difference between different groups. Spearman's correlation test was used to analyze the relationship between the levels of CXCL10 and the clinical periodontal parameters. -value of ≤0.05 was considered significant.
Significantly higher concentrations of CXCL10 were found in saliva and serum in chronic periodontitis patients as compared with the controls (272±60.4 pg/mL and 72±13.4 pg/mL vs 130±22.2 pg/mL and 44.08±4.5 pg/mL, ≤0.05). The CXCL10 levels in GCF were higher in the periodontitis group as compared with the control group (66.36±32.0 pg/mL and 44.56±17.5 pg/mL, respectively); the difference did not reach statistical significance (≥0.05). Moreover, serum CXCL10 level was significantly higher in periodontitis patients with moderate to severe bone loss as compared with those with mild bone loss (71.05±4.7 pg/mL vs 54.8±7.7 pg/mL, ≤0.05). The serum CXCL10 levels were found to be related to CAL measurements (=0.3, =0.026), while the saliva CXCL10 levels were related to PD measurements (=0.8, =0.0007).
CXCL10 is significantly increased in periodontitis subjects as compared with controls and could be used as a marker for periodontal disease.
本研究旨在通过测定牙周健康对照受试者和慢性牙周炎成年受试者的唾液、血清和龈沟液（GCF）样本中的CXCL10水平，评估CXCL10作为牙周炎生物标志物的价值。
在乌姆古拉大学牙科诊所寻求牙科治疗的成年患者接受了全面的牙周检查，并收集了唾液、血清和GCF样本。如果受试者超过30%的牙齿牙周探诊深度（PD）≥4mm和/或临床附着水平（CAL）≥3mm，则被分类为慢性牙周炎患者（n = 31）。对照组（n = 25）的PD≤3mm和/或CAL≤2mm。采用酶联免疫吸附测定（ELISA）法测定唾液、血清和GCF样本中CXCL10的浓度。采用学生t检验评估不同组之间的显著差异。采用Spearman相关性检验分析CXCL10水平与临床牙周参数之间的关系。P值≤0.05被认为具有显著性。
与对照组相比，慢性牙周炎患者唾液和血清中CXCL10的浓度显著更高（分别为272±60.4 pg/mL和72±13.4 pg/mL，而对照组为130±22.2 pg/mL和44.08±4.5 pg/mL，P≤0.05）。牙周炎组GCF中的CXCL10水平高于对照组（分别为66.36±32.0 pg/mL和44.56±17.5 pg/mL）；差异未达到统计学显著性（P≥0.05）。此外,与轻度骨丧失的牙周炎患者相比，中度至重度骨丧失的牙周炎患者血清CXCL10水平显著更高（71.05±4.7 pg/mL对54.8±7.7 pg/mL，P≤0.05）。发现血清CXCL10水平与CAL测量值相关（r = 0.3，P = 0.026），而唾液CXCL10水平与PD测量值相关（r = 0.8，P = 0.0007）。
与对照组相比，牙周炎受试者中CXCL10显著升高，可作为牙周疾病的标志物。
