College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan 430070, China.
Food Funct. 2022 May 23;13(10):5626-5639. doi: 10.1039/d1fo04004a.
This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice. During the experimental period, mice were administered 200 mg kg or 600 mg kg KOS, 200 mg kg sulfasalazine and a combination of KOS and sulfasalazine for 14 days. The mice were then treated with drinking water containing 2.5% DSS for 9 days, while the intervention of KOS and sulfasalazine continued. At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated. The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins ( < 0.05). The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal. Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment. The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (, and ) and up-regulates the abundance of anti-inflammatory bacteria ( and ). These findings provide new insights into dietary management for patients with inflammatory bowel disease.
本研究旨在探讨魔芋低聚糖（KOS）对结肠炎小鼠炎症性肠病的保护作用。实验期间，各组小鼠分别灌胃给予 200 mg·kg -1 、600 mg·kg -1 的 KOS，200 mg·kg -1 的柳氮磺胺吡啶，以及 KOS 与柳氮磺胺吡啶联合用药，连续给药 14 d。然后，各组小鼠饮用含 2.5% DSS 的水 9 d，同时继续给予 KOS 和柳氮磺胺吡啶干预。实验结束时，评估小鼠的表型、结肠组织的病理损伤、细胞因子参数和肠道微生物群。结果表明，KOS 处理的小鼠结肠组织的病理损伤减轻，紧密连接蛋白的表达显著增加（<0.05）。结肠炎小鼠结肠组织中炎性细胞因子的水平趋于正常。此外，肠道微生物群分析表明，KOS 处理还调节了肠道微生物的结构和组成。其可能的内在机制是 KOS 下调了促炎菌（、和）的丰度，上调了抗炎菌（和）的丰度。这些发现为炎症性肠病患者的饮食管理提供了新的思路。
