Vedanarayanan V V, Kaplan B S, Fong J S
Pediatr Res. 1987 Mar;21(3):252-6. doi: 10.1203/00006450-198703000-00009.
To understand the role of neutrophil leukocytosis in hemolytic uremic syndrome, we studied the changes in neutrophil function in the modified generalized Shwartzman reaction in rabbits. This model resembles hemolytic uremic syndrome associated with endotoxemia. At the end of an endotoxin infusion, we observed leukopenia, thrombocytopenia, and a decrease in hematocrit associated with schistocytosis. Plasma B-glucuronidase levels increased and this was associated with a decrease in neutrophil content of the enzyme. The chemotactic index and neutrophil aggregation to zymosan-activated serum were impaired compared to controls. The neutrophil procoagulant content increased after endotoxin infusion. The serum creatinine concentration and proteinuria increased in the endotoxin-treated animals. The changes returned to normal by 48 h. Renal cortical malondialdehyde, a reflection of lipid peroxidation, was higher in the endotoxin-treated animals than in the controls. We have shown enzyme release by neutrophils, impairment of chemotaxis and aggregation, increased procoagulant content in neutrophils, and evidence of lipid peroxidation in renal cortical tissue in this model. These observations raise the possibility that leukocytes may have a role in the pathogenesis of the hemolytic uremic syndrome.
为了解中性粒细胞增多在溶血尿毒综合征中的作用，我们研究了家兔改良全身性施瓦茨曼反应中中性粒细胞功能的变化。该模型类似于与内毒素血症相关的溶血尿毒综合征。在内毒素输注结束时，我们观察到白细胞减少、血小板减少以及与裂体细胞症相关的血细胞比容降低。血浆β-葡萄糖醛酸酶水平升高，这与该酶在中性粒细胞中的含量降低有关。与对照组相比，趋化指数以及中性粒细胞对酵母聚糖激活血清的聚集能力均受损。内毒素输注后中性粒细胞促凝物质含量增加。内毒素处理的动物血清肌酐浓度和蛋白尿增加。这些变化在48小时后恢复正常。作为脂质过氧化反映的肾皮质丙二醛在内毒素处理的动物中高于对照组。在该模型中，我们已证明中性粒细胞会释放酶、趋化性和聚集功能受损、中性粒细胞促凝物质含量增加以及肾皮质组织中存在脂质过氧化的证据。这些观察结果提示白细胞可能在溶血尿毒综合征的发病机制中起作用。
