Bergstein J M
Lab Invest. 1976 Aug;35(2):171-8.
A fibrin slide test was utilized to study the effect of platelets and endotoxin on renal cortical fibrinolysis in rabbits. Cortical lysis was absent in kidney from animals made markedly thrombocytopenic with goat antirabbit platelet serum or endotoxin; light microscopy was normal and immunofluorescent microscopy for fibrin was negative. No loss of lysis was detected in kidney of animals given goat anti-rabbit albumin or isotonic saline. Plasma from animals with endotoxin or antiplatelet antibody-induced thrombocytopenia demonstrated an increased capacity to inhibit cortical fibrinolytic activity of normal rabbit kidney. Antiplatelet antibody could be substituted for the preparatory injection of endotoxin in the generalized Shwartzman reaction; a parallel rise in lysis inhibitory titer was detected in plasma from animals prepared with antibody or endotoxin. Although lysis was absent following incubation of normal renal cortex with washed platelets, no inhibition of lysis was found after incubation of normal cortex with endotoxin, histamine, serotonin, platelet membranes, or granules. Using a modified fibrin slide technique, a diffusable platelet inhibitor of lysis could be demonstrated. These studies indicate that platelets contain an inhibitor to renal cortical fibrinolysis which is released into the circulation upon platelet destruction. The findings also suggest that platelet destruction is the mechanism through which endotoxin inhibits cortical lysis.
采用纤维蛋白玻片试验研究血小板和内毒素对兔肾皮质纤维蛋白溶解的影响。用山羊抗兔血小板血清或内毒素使动物明显血小板减少，其肾脏皮质无溶解现象；光学显微镜检查正常，纤维蛋白免疫荧光显微镜检查为阴性。给动物注射山羊抗兔白蛋白或等渗盐水，其肾脏未检测到溶解能力丧失。内毒素或抗血小板抗体诱导血小板减少的动物血浆，对正常兔肾皮质纤维蛋白溶解活性的抑制能力增强。在全身性施瓦茨曼反应中，抗血小板抗体可替代内毒素的预注射；用抗体或内毒素处理的动物血浆中，溶解抑制效价呈平行升高。虽然正常肾皮质与洗涤后的血小板孵育后无溶解现象，但正常皮质与内毒素、组胺、5-羟色胺、血小板膜或颗粒孵育后未发现溶解受抑制。采用改良的纤维蛋白玻片技术，可证明存在一种可扩散的血小板溶解抑制剂。这些研究表明，血小板含有一种肾皮质纤维蛋白溶解抑制剂，血小板破坏时该抑制剂释放到循环中。研究结果还提示，血小板破坏是内毒素抑制皮质溶解的机制。
